Combined use of gliadins and SSRs to analyse the genetic variability of the Spanish collection of cultivated diploid wheat (Triticum monococcum L. ssp. monococcum)

Ruiz Valcárcel, Magdalena and Aguiriano Labandibar, Edurne and Fité Sánchez, Mª del Rosario and Carrillo Becerril, Jose Maria (2007). Combined use of gliadins and SSRs to analyse the genetic variability of the Spanish collection of cultivated diploid wheat (Triticum monococcum L. ssp. monococcum). "Genetic Resources And Crop Evolution", v. 54 (n. 8); pp. 1849-1860. ISSN 0925-9864. https://doi.org/10.1007/s10722-007-9208-7.

Description

Title: Combined use of gliadins and SSRs to analyse the genetic variability of the Spanish collection of cultivated diploid wheat (Triticum monococcum L. ssp. monococcum)
Author/s:
  • Ruiz Valcárcel, Magdalena
  • Aguiriano Labandibar, Edurne
  • Fité Sánchez, Mª del Rosario
  • Carrillo Becerril, Jose Maria
Item Type: Article
Título de Revista/Publicación: Genetic Resources And Crop Evolution
Date: December 2007
ISSN: 0925-9864
Volume: 54
Subjects:
Freetext Keywords: Genetic variability - Germplasm - Gliadin alleles - Microsatellites - Triticum monococcum L.
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

This work studied the combined use of gliadins and SSRs to analyse inter- and intra-accession variability of the Spanish collection of cultivated einkorn (Triticum monococcum L. ssp. monococcum) maintained at the CRF-INIA. In general, gliadin loci presented higher discrimination power than SSRs, reflecting the high variability of the gliadins. The loci on chromosome 6A were the most polymorphic with similar PIC values for both marker systems, showing that these markers are very useful for genetic variability studies in wheat. The gliadin results indicated that the Spanish einkorn collection possessed high genetic diversity, being the differentiation large between varieties and small within them. Some associations between gliadin alleles and geographical and agro-morphological data were found. Agro-morphological relations were also observed in the clusters of the SSRs dendrogram. A high concordance was found between gliadins and SSRs for genotype identification. In addition, both systems provide complementary information to resolve the different cases of intra-accession variability not detected at the agro-morphological level, and to identify separately all the genotypes analysed. The combined use of both genetic markers is an excellent tool for genetic resource evaluation in addition to agro-morphological evaluation.

More information

Item ID: 10989
DC Identifier: http://oa.upm.es/10989/
OAI Identifier: oai:oa.upm.es:10989
DOI: 10.1007/s10722-007-9208-7
Official URL: http://www.springerlink.com/content/8qx5312751n27424/
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2012 10:20
Last Modified: 20 Apr 2016 19:10
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