Genetic redundancy among durum wheat accessions as assessed with SSRs and endosperm proteins

Ruiz Valcárcel, Magdalena and Aguiriano Labandibar, Edurne and Giraldo Carbajo, Patricia and Carrillo Becerril, Jose Maria (2011). Genetic redundancy among durum wheat accessions as assessed with SSRs and endosperm proteins. "Spanish Journal of Agricultural Research", v. 9 (n. 1); pp. 156-165. ISSN 1695-971-X.

Description

Title: Genetic redundancy among durum wheat accessions as assessed with SSRs and endosperm proteins
Author/s:
  • Ruiz Valcárcel, Magdalena
  • Aguiriano Labandibar, Edurne
  • Giraldo Carbajo, Patricia
  • Carrillo Becerril, Jose Maria
Item Type: Article
Título de Revista/Publicación: Spanish Journal of Agricultural Research
Date: 2011
ISSN: 1695-971-X
Volume: 9
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Reducing duplication in ex-situ collections is complicated and requires good quality genetic markers. This study was conducted to assess the value of endosperm proteins and SSRs for validation of potential duplicates and monitoring intra-accession variability. Fifty durum wheat (Triticum turgidum ssp. durum) accessions grouped in 23 potential duplicates, and previously characterised for 30 agro-morphological traits, were analysed for gliadin and high molecular weight glutenin (HMWG) subunit alleles, total protein, and 24 SSRs, covering a wide genome area. Similarity and dissimilarity matrices were generated based on protein and SSRs alleles. For heterogeneous accessions at gliadins the percent pattern homology (PH) between gliadin patterns and the Nei’s coefficient of genetic identity (I) were computed. Eighteen duplicates identical for proteins showed none or less than 3 unshared SSRs alleles. For heterogeneous accessions PH and I values lower than 80 identified clearly off-types with more than 3 SSRs unshared. Only those biotypes differing in no more than one protein-coding locus were confirmed with SSRs. A good concordance among proteins, morphological traits, and SSR were detected. However, the discrepancy in similarity detected in some cases showed that it is advisable to evaluate redundancy through distinct approaches. The analysis in proteins together with SSRs data are very useful to identify duplicates, biotypes, close related genotypes, and contaminations

More information

Item ID: 10994
DC Identifier: http://oa.upm.es/10994/
OAI Identifier: oai:oa.upm.es:10994
Official URL: http://revistas.inia.es/index.php/sjar/article/view/1541
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2012 09:57
Last Modified: 20 Apr 2016 19:10
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