Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi

Delgado Cerezo, María Magdalena and Sánchez Rodríguez, Clara and Escudero Welsch, Viviana and Miedes Vicente, Eva and Virginia Fernandez, Paula and Jordá Miró, Lucía and Hernandez Blanco, Camilo and Sánchez Vallet, Andrea and Bednarek, Pawel and Schulze-Lefert, Paul and Somerville, Shauna and Estevez, José Manuel and Persson, Staffan and Molina Fernández, Antonio (2012). Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi. "Molecular Plant", v. 5 (n. 1); pp. 98-114. ISSN 1674-2052. https://doi.org/10.1093/mp/ssr082.

Description

Title: Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
Author/s:
  • Delgado Cerezo, María Magdalena
  • Sánchez Rodríguez, Clara
  • Escudero Welsch, Viviana
  • Miedes Vicente, Eva
  • Virginia Fernandez, Paula
  • Jordá Miró, Lucía
  • Hernandez Blanco, Camilo
  • Sánchez Vallet, Andrea
  • Bednarek, Pawel
  • Schulze-Lefert, Paul
  • Somerville, Shauna
  • Estevez, José Manuel
  • Persson, Staffan
  • Molina Fernández, Antonio
Item Type: Article
Título de Revista/Publicación: Molecular Plant
Date: January 2012
ISSN: 1674-2052
Volume: 5
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition.

More information

Item ID: 11406
DC Identifier: http://oa.upm.es/11406/
OAI Identifier: oai:oa.upm.es:11406
DOI: 10.1093/mp/ssr082
Official URL: http://mplant.oxfordjournals.org/content/5/1/98.abstract
Deposited by: Memoria Investigacion
Deposited on: 25 Jul 2012 10:41
Last Modified: 20 Apr 2016 19:29
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