Processing Pipeline for Digitalizing the Lineage Tree of Early Zebrafish Embryogenesis from Multiharmonic Imaging

Luengo Oroz, Miguel Ángel and Savy, Thierry and Rubio, Jose L. and Duloquin, Louise and Faure, Emmanuel and Olivier, Nicolas and Ledesma Carbayo, María Jesús and Debarre, Delphine and Bourgine, Paul and Beaurepaire, Emmanuel and Peyrieras, Nadine and Santos Lleo, Andres de (2011). Processing Pipeline for Digitalizing the Lineage Tree of Early Zebrafish Embryogenesis from Multiharmonic Imaging. In: "IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011)", 30/03/2011 - 02/04/2011, Chicago, IL, EEUU. ISBN 978-1-4244-4127-3.

Description

Title: Processing Pipeline for Digitalizing the Lineage Tree of Early Zebrafish Embryogenesis from Multiharmonic Imaging
Author/s:
  • Luengo Oroz, Miguel Ángel
  • Savy, Thierry
  • Rubio, Jose L.
  • Duloquin, Louise
  • Faure, Emmanuel
  • Olivier, Nicolas
  • Ledesma Carbayo, María Jesús
  • Debarre, Delphine
  • Bourgine, Paul
  • Beaurepaire, Emmanuel
  • Peyrieras, Nadine
  • Santos Lleo, Andres de
Item Type: Presentation at Congress or Conference (Article)
Event Title: IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011)
Event Dates: 30/03/2011 - 02/04/2011
Event Location: Chicago, IL, EEUU
Title of Book: Proceedings of IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011)
Date: 2011
ISBN: 978-1-4244-4127-3
Subjects:
Faculty: E.T.S.I. Telecomunicación (UPM)
Department: Ingeniería Electrónica
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The reconstruction of the cell lineage tree of early zebrafish embryogenesis requires the use of in-vivo microscopy imaging and image processing strategies. Second (SHG) and third harmonic generation (THG) microscopy observations in unstained zebrafish embryos allows to detect cell divisions and cell membranes from 1-cell to 1K-cell stage. In this article, we present an ad-hoc image processing pipeline for cell tracking and cell membranes segmentation enabling the reconstruction of the early zebrafish cell lineage tree until the 1K-cell stage. This methodology has been used to obtain digital zebrafish embryos allowing to generate a quantitative description of early zebrafish embryogenesis with minute temporal accuracy and μm spatial resolution

More information

Item ID: 12041
DC Identifier: http://oa.upm.es/12041/
OAI Identifier: oai:oa.upm.es:12041
Official URL: http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5872699&tag=1
Deposited by: Memoria Investigacion
Deposited on: 17 Sep 2012 11:04
Last Modified: 21 Apr 2016 11:12
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