Luengo Oroz, Miguel Ángel and Savy, Thierry and Rubio, Jose L. and Duloquin, Louise and Faure, Emmanuel and Olivier, Nicolas and Ledesma Carbayo, María Jesús and Debarre, Delphine and Bourgine, Paul and Beaurepaire, Emmanuel and Peyrieras, Nadine and Santos Lleo, Andres de
Processing Pipeline for Digitalizing the Lineage Tree of Early Zebrafish Embryogenesis from Multiharmonic Imaging.
In: "IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI 2011)", 30/03/2011 - 02/04/2011, Chicago, IL, EEUU. ISBN 978-1-4244-4127-3.
The reconstruction of the cell lineage tree of early zebrafish embryogenesis requires the use of in-vivo microscopy imaging and image processing strategies. Second (SHG) and third harmonic generation (THG) microscopy observations in unstained zebrafish embryos allows to detect cell divisions and cell membranes from 1-cell to 1K-cell stage. In this article, we present an ad-hoc image processing pipeline for cell tracking and cell membranes segmentation enabling the reconstruction of the early zebrafish cell lineage tree until the 1K-cell stage. This methodology has been used to obtain digital zebrafish embryos allowing to generate a quantitative description of early zebrafish embryogenesis with minute temporal accuracy and μm spatial resolution