Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana

Samol, Iga; Buhr, Frank; Springer, Armin; Pollmann, Stephan; Lahroussi, Abder; Rossig, Claudia; von Wettstein, Diter; Reinbothe, Christiane y Reinbothe, Steffen (2011). Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana. "Plant And Cell Physiology", v. 52 (n. 1); pp. 84-95. ISSN 0032-0781. https://doi.org/10.1093/pcp/pcq176.

Descripción

Título: Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana
Autor/es:
  • Samol, Iga
  • Buhr, Frank
  • Springer, Armin
  • Pollmann, Stephan
  • Lahroussi, Abder
  • Rossig, Claudia
  • von Wettstein, Diter
  • Reinbothe, Christiane
  • Reinbothe, Steffen
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant And Cell Physiology
Fecha: 2011
Volumen: 52
Materias:
Escuela: Otros Centros UPM
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Singlet oxygen is a prominent form of reactive oxygen species in higher plants. It is easily formed from molecular oxygen by triplet–triplet interchange with excited porphyrin species. Evidence has been obtained from studies on the flu mutant of Arabidopsis thaliana of a genetically determined cell death pathway that involves differential changes at the transcriptome level. Here we report on a different cell death pathway that can be deduced from the analysis of oep16 mutants of A. thaliana. Pure lines of four independent OEP16-deficient mutants with different cell death properties were isolated. Two of the mutants overproduced free protochlorophyllide (Pchlide) in the dark because of defects in import of NADPH:Pchlide oxidoreductase A (pPORA) and died after illumination. The other two mutants avoided excess Pchlide accumulation. Using pulse labeling and polysome profiling studies we show that translation is a major site of cell death regulation in flu and oep16 plants. flu plants respond to photooxidative stress triggered by singlet oxygen by reprogramming their translation toward synthesis of key enzymes involved in jasmonic acid synthesis and stress proteins. In contrast, those oep16 mutants that were prone to photooxidative damage were unable to respond in this way. Together, our results show that translation is differentially affected in the flu and oep16 mutants in response to singlet oxygen.

Más información

ID de Registro: 12158
Identificador DC: http://oa.upm.es/12158/
Identificador OAI: oai:oa.upm.es:12158
Identificador DOI: 10.1093/pcp/pcq176
URL Oficial: http://pcp.oxfordjournals.org/content/52/1/84.long
Depositado por: Memoria Investigacion
Depositado el: 10 Sep 2012 07:48
Ultima Modificación: 21 Abr 2016 11:21
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