Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana

Samol, Iga and Buhr, Frank and Springer, Armin and Pollmann, Stephan and Lahroussi, Abder and Rossig, Claudia and von Wettstein, Diter and Reinbothe, Christiane and Reinbothe, Steffen (2011). Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana. "Plant And Cell Physiology", v. 52 (n. 1); pp. 84-95. ISSN 0032-0781. https://doi.org/10.1093/pcp/pcq176.

Description

Title: Implication of the oep16-1 mutation in a flu-independent, singlet oxygen-regulated cell death pathway in Arabidopsis thaliana
Author/s:
  • Samol, Iga
  • Buhr, Frank
  • Springer, Armin
  • Pollmann, Stephan
  • Lahroussi, Abder
  • Rossig, Claudia
  • von Wettstein, Diter
  • Reinbothe, Christiane
  • Reinbothe, Steffen
Item Type: Article
Título de Revista/Publicación: Plant And Cell Physiology
Date: 2011
Volume: 52
Subjects:
Faculty: Otros Centros UPM
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB) | Preview

Abstract

Singlet oxygen is a prominent form of reactive oxygen species in higher plants. It is easily formed from molecular oxygen by triplet–triplet interchange with excited porphyrin species. Evidence has been obtained from studies on the flu mutant of Arabidopsis thaliana of a genetically determined cell death pathway that involves differential changes at the transcriptome level. Here we report on a different cell death pathway that can be deduced from the analysis of oep16 mutants of A. thaliana. Pure lines of four independent OEP16-deficient mutants with different cell death properties were isolated. Two of the mutants overproduced free protochlorophyllide (Pchlide) in the dark because of defects in import of NADPH:Pchlide oxidoreductase A (pPORA) and died after illumination. The other two mutants avoided excess Pchlide accumulation. Using pulse labeling and polysome profiling studies we show that translation is a major site of cell death regulation in flu and oep16 plants. flu plants respond to photooxidative stress triggered by singlet oxygen by reprogramming their translation toward synthesis of key enzymes involved in jasmonic acid synthesis and stress proteins. In contrast, those oep16 mutants that were prone to photooxidative damage were unable to respond in this way. Together, our results show that translation is differentially affected in the flu and oep16 mutants in response to singlet oxygen.

More information

Item ID: 12158
DC Identifier: http://oa.upm.es/12158/
OAI Identifier: oai:oa.upm.es:12158
DOI: 10.1093/pcp/pcq176
Official URL: http://pcp.oxfordjournals.org/content/52/1/84.long
Deposited by: Memoria Investigacion
Deposited on: 10 Sep 2012 07:48
Last Modified: 21 Apr 2016 11:21
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM