Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase a-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?

Man, Huimin; Pollmann, Stephan; Weiler, Elmar W. y Kirby, Edward G. (2011). Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase a-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?. "Journal of Experimental Botany", v. 62 (n. 13); pp. 4423-4431. ISSN 0022-0957. https://doi.org/10.1093/jxb/err026.

Descripción

Título: Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase a-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?
Autor/es:
  • Man, Huimin
  • Pollmann, Stephan
  • Weiler, Elmar W.
  • Kirby, Edward G.
Tipo de Documento: Artículo
Título de Revista/Publicación: Journal of Experimental Botany
Fecha: 2011
Volumen: 62
Materias:
Escuela: Otros Centros UPM
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The initial reaction in the pathway leading to the production of indole-3-acetic acid (IAA) in plants is the reaction between chorismate and glutamine to produce anthranilate, catalysed by the enzyme anthranilate synthase (ASA; EC 4.1.3.27). Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine and significantly enhanced ASA a-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively). Similarly, tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively). Furthermore, free IAA was significantly elevated both in leaves of GS1a transgenic poplar and in tobacco leaves fed with 30 mM glutamine and 2 mM chorismate. These results indicated that enhanced cellular glutamine may account for the enhanced growth in GS transgenic poplars through the regulation of auxin biosynthesis

Más información

ID de Registro: 12163
Identificador DC: http://oa.upm.es/12163/
Identificador OAI: oai:oa.upm.es:12163
Identificador DOI: 10.1093/jxb/err026
Depositado por: Memoria Investigacion
Depositado el: 05 Sep 2012 11:19
Ultima Modificación: 21 Abr 2016 11:21
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