Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase a-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?

Man, Huimin and Pollmann, Stephan and Weiler, Elmar W. and Kirby, Edward G. (2011). Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase a-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?. "Journal of Experimental Botany", v. 62 (n. 13); pp. 4423-4431. ISSN 0022-0957. https://doi.org/10.1093/jxb/err026.

Description

Title: Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase a-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?
Author/s:
  • Man, Huimin
  • Pollmann, Stephan
  • Weiler, Elmar W.
  • Kirby, Edward G.
Item Type: Article
Título de Revista/Publicación: Journal of Experimental Botany
Date: 2011
ISSN: 0022-0957
Volume: 62
Subjects:
Faculty: Otros Centros UPM
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The initial reaction in the pathway leading to the production of indole-3-acetic acid (IAA) in plants is the reaction between chorismate and glutamine to produce anthranilate, catalysed by the enzyme anthranilate synthase (ASA; EC 4.1.3.27). Compared with non-transgenic controls, leaves of transgenic poplar with ectopic expression of the pine cytosolic glutamine synthetase (GS1a; EC 6.3.1.2) produced significantly greater glutamine and significantly enhanced ASA a-subunit (ASA1) transcript and protein (approximately 130% and 120% higher than in the untransformed controls, respectively). Similarly, tobacco leaves fed with 30 mM glutamine and 2 mM chorismate showed enhanced ASA1 transcript and protein (175% and 90% higher than controls, respectively). Furthermore, free IAA was significantly elevated both in leaves of GS1a transgenic poplar and in tobacco leaves fed with 30 mM glutamine and 2 mM chorismate. These results indicated that enhanced cellular glutamine may account for the enhanced growth in GS transgenic poplars through the regulation of auxin biosynthesis

More information

Item ID: 12163
DC Identifier: http://oa.upm.es/12163/
OAI Identifier: oai:oa.upm.es:12163
DOI: 10.1093/jxb/err026
Deposited by: Memoria Investigacion
Deposited on: 05 Sep 2012 11:19
Last Modified: 21 Apr 2016 11:21
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