Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

Meneghetti, Stefano; Costacurta, Angelo; Frare, Enrica; Da Rold, Graziana; Migliaro, Daniele; Morreale, Giacomo; Crespan, Manna; Sotés Ruiz, Vicente y Calo, Antonio (2011). Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems. "Molecular Biotechnology", v. 48 (n. 3); pp. 244-254. ISSN 1073-6085. https://doi.org/10.1007/s12033-010-9365-3.

Descripción

Título: Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems
Autor/es:
  • Meneghetti, Stefano
  • Costacurta, Angelo
  • Frare, Enrica
  • Da Rold, Graziana
  • Migliaro, Daniele
  • Morreale, Giacomo
  • Crespan, Manna
  • Sotés Ruiz, Vicente
  • Calo, Antonio
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Biotechnology
Fecha: Julio 2011
Volumen: 48
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Producción Vegetal: Fitotecnia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia province; Cannonau in Sardinia), 19 Spanish accessions of different types (named Garnacha tinta, Garnacha blanca, Garnacha peluda, Garnacha roja, Garnacha erguida, Garnacha roya) and 6 French accessions (named Grenache and Grenache noir). In order to verify the varietal identity of the samples, analyses based on 14 simple sequence repeat (SSR) loci were performed. The presence of an additional allele at ISV3 locus (151 bp) was found in four Tocai rosso accessions and in a Sardinian Cannonau clone, that are, incidentally, chimeras. In addition to microsatellite analysis, intravarietal variability study was performed using AFLP, SAMPL and M-AFLP molecular markers. AFLPs could discriminate among several Garnacha samples; SAMPLs allowed distinguishing few genotypes on the basis of their geographic origin, whereas M-AFLPs revealed plant-specific markers, differentiating all accessions. Italian samples showed the greatest variability among themselves, especially on the basis of their different provenance, while Spanish samples were the most similar, in spite of their morphological diversity.

Más información

ID de Registro: 13640
Identificador DC: http://oa.upm.es/13640/
Identificador OAI: oai:oa.upm.es:13640
Identificador DOI: 10.1007/s12033-010-9365-3
URL Oficial: http://www.springerlink.com/content/r76577102v1004w2/
Depositado por: Memoria Investigacion
Depositado el: 11 Oct 2012 10:11
Ultima Modificación: 21 Abr 2016 12:58
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