Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

Meneghetti, Stefano and Costacurta, Angelo and Frare, Enrica and Da Rold, Graziana and Migliaro, Daniele and Morreale, Giacomo and Crespan, Manna and Sotés Ruiz, Vicente and Calo, Antonio (2011). Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems. "Molecular Biotechnology", v. 48 (n. 3); pp. 244-254. ISSN 1073-6085. https://doi.org/10.1007/s12033-010-9365-3.

Description

Title: Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems
Author/s:
  • Meneghetti, Stefano
  • Costacurta, Angelo
  • Frare, Enrica
  • Da Rold, Graziana
  • Migliaro, Daniele
  • Morreale, Giacomo
  • Crespan, Manna
  • Sotés Ruiz, Vicente
  • Calo, Antonio
Item Type: Article
Título de Revista/Publicación: Molecular Biotechnology
Date: July 2011
ISSN: 1073-6085
Volume: 48
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Vegetal: Fitotecnia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview

Abstract

This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and Spain. The samples studied include 28 Italian accessions (named Tocai rosso in Vicenza area; Alicante in Sicily and Elba island; Gamay perugino in Perugia province; Cannonau in Sardinia), 19 Spanish accessions of different types (named Garnacha tinta, Garnacha blanca, Garnacha peluda, Garnacha roja, Garnacha erguida, Garnacha roya) and 6 French accessions (named Grenache and Grenache noir). In order to verify the varietal identity of the samples, analyses based on 14 simple sequence repeat (SSR) loci were performed. The presence of an additional allele at ISV3 locus (151 bp) was found in four Tocai rosso accessions and in a Sardinian Cannonau clone, that are, incidentally, chimeras. In addition to microsatellite analysis, intravarietal variability study was performed using AFLP, SAMPL and M-AFLP molecular markers. AFLPs could discriminate among several Garnacha samples; SAMPLs allowed distinguishing few genotypes on the basis of their geographic origin, whereas M-AFLPs revealed plant-specific markers, differentiating all accessions. Italian samples showed the greatest variability among themselves, especially on the basis of their different provenance, while Spanish samples were the most similar, in spite of their morphological diversity.

More information

Item ID: 13640
DC Identifier: http://oa.upm.es/13640/
OAI Identifier: oai:oa.upm.es:13640
DOI: 10.1007/s12033-010-9365-3
Official URL: http://www.springerlink.com/content/r76577102v1004w2/
Deposited by: Memoria Investigacion
Deposited on: 11 Oct 2012 10:11
Last Modified: 21 Apr 2016 12:58
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM