Gene Expression Profiling in Limb-Girdle Muscular Dystrophy 2A

Sáenz, Amets; Azpitarte, Margarita; Armañanzas Arnedillo, Ruben; Leturcq, France; Alzualde, Ainhoa; Inza Cano, Iñaki; García Bragado, Federico; Herran, Gaspar de la; Corcuera, Julian; Cabello, Ana; Navarro, Carmen; Torre, Carolina de la; Gallardo, Eduard; Illa, Isabel y López de Munain, Adolfo (2008). Gene Expression Profiling in Limb-Girdle Muscular Dystrophy 2A. "Plos One", v. 3 (n. 11); pp. 1-14. ISSN 1932-6203. https://doi.org/10.1371/journal.pone.0003750.

Descripción

Título: Gene Expression Profiling in Limb-Girdle Muscular Dystrophy 2A
Autor/es:
  • Sáenz, Amets
  • Azpitarte, Margarita
  • Armañanzas Arnedillo, Ruben
  • Leturcq, France
  • Alzualde, Ainhoa
  • Inza Cano, Iñaki
  • García Bragado, Federico
  • Herran, Gaspar de la
  • Corcuera, Julian
  • Cabello, Ana
  • Navarro, Carmen
  • Torre, Carolina de la
  • Gallardo, Eduard
  • Illa, Isabel
  • López de Munain, Adolfo
Tipo de Documento: Artículo
Título de Revista/Publicación: Plos One
Fecha: 2008
Volumen: 3
Materias:
Escuela: Facultad de Informática (UPM) [antigua denominación]
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Limb-girdle muscular dystrophy type 2A (LGMD2A) is a recessive genetic disorder caused by mutations in calpain 3 (CAPN3). Calpain 3 plays different roles in muscular cells, but little is known about its functions or in vivo substrates. The aim of this study was to identify the genes showing an altered expression in LGMD2A patients and the possible pathways they are implicated in. Ten muscle samples from LGMD2A patients with in which molecular diagnosis was ascertained were investigated using array technology to analyze gene expression profiling as compared to ten normal muscle samples. Upregulated genes were mostly those related to extracellular matrix (different collagens), cell adhesion (fibronectin), muscle development (myosins and melusin) and signal transduction. It is therefore suggested that different proteins located or participating in the costameric region are implicated in processes regulated by calpain 3 during skeletal muscle development. Genes participating in the ubiquitin proteasome degradation pathway were found to be deregulated in LGMD2A patients, suggesting that regulation of this pathway may be under the control of calpain 3 activity. As frizzled-related protein (FRZB) is upregulated in LGMD2A muscle samples, it could be hypothesized that β-catenin regulation is also altered at the Wnt signaling pathway, leading to an incorrect myogenesis. Conversely, expression of most transcription factor genes was downregulated (MYC, FOS and EGR1). Finally, the upregulation of IL-32 and immunoglobulin genes may induce the eosinophil chemoattraction explaining the inflammatory findings observed in presymptomatic stages. The obtained results try to shed some light on identification of novel therapeutic targets for limb-girdle muscular dystrophies

Más información

ID de Registro: 13980
Identificador DC: http://oa.upm.es/13980/
Identificador OAI: oai:oa.upm.es:13980
Identificador DOI: 10.1371/journal.pone.0003750
URL Oficial: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003750
Depositado por: Memoria Investigacion
Depositado el: 21 Dic 2012 10:30
Ultima Modificación: 21 Abr 2016 13:26
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