Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid

Pollmann, Stephan and Neu, Daniel and Weiler, Elmar W. (2003). Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid. "Phytochemistry", v. 62 (n. 3); pp. 293-300. ISSN 0031-9422. https://doi.org/10.1016/S0031-9422(02)00563-0.

Description

Title: Molecular cloning and characterization of an amidase from Arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid
Author/s:
  • Pollmann, Stephan
  • Neu, Daniel
  • Weiler, Elmar W.
Item Type: Article
Título de Revista/Publicación: Phytochemistry
Date: 2003
ISSN: 0031-9422
Volume: 62
Subjects:
Freetext Keywords: Arabidopsis thaliana; Brassicaceae; Crucifers; Indole-3-acetic acid; Indole-3-acetamide; Indole-3-acetonitrile; Nitrilases; Acylamide amidohydrolase; Amidase
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Acylamidohydrolases from higher plants have not been characterized or cloned so far. AtAMI1 is the first member of this enzyme family from a higher plant and was identified in the genome of Arabidopsis thaliana based on sequence homology with the catalytic-domain sequence of bacterial acylamidohydrolases, particularly those that exhibit indole-3-acetamide amidohydrolase activity. AtAMI1 polypeptide and mRNA are present in leaf tissues, as shown by immunoblotting and RT-PCR, respectively. AtAMI1 was expressed from its cDNA in enzymatically active form and exhibits substrate specificity for indole-3-acetamide, but also some activity against l-asparagine. The recombinant enzyme was characterized further. The results show that higher plants have acylamidohydrolases with properties similar to the enzymes of certain plant-associated bacteria such as Agrobacterium-, Pseudomonas- and Rhodococcus-species, in which these enzymes serve to synthesize the plant growth hormone, indole-3-acetic acid, utilized by the bacteria to colonize their host plants. As indole-3-acetamide is a native metabolite in Arabidopsis thaliana, it can no longer be ruled out that one pathway for the biosynthesis of indole-3-acetic acid involves indole-3-acetamide-hydrolysis by AtAMI1.

More information

Item ID: 14040
DC Identifier: http://oa.upm.es/14040/
OAI Identifier: oai:oa.upm.es:14040
DOI: 10.1016/S0031-9422(02)00563-0
Official URL: http://www.sciencedirect.com/science/article/pii/S0031942202005630
Deposited by: Memoria Investigacion
Deposited on: 20 Dec 2012 18:37
Last Modified: 21 Apr 2016 13:31
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