Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones

Ortiz Marcide, Jesus Maria and Martin Clemente, Juan Pedro and Castro, Isaura and D'Ofrio, Claudio and De Lorenzi, Gabriela and Ferreira, Vanessa and Pinto-Carnide, Olinda (2012). Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones. "Molecular Biotechnology", v. 52 (n. 1); pp. 26-39. ISSN 1073-6085. https://doi.org/10.1007/s12033-011-9470-y.

Description

Title: Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones
Author/s:
  • Ortiz Marcide, Jesus Maria
  • Martin Clemente, Juan Pedro
  • Castro, Isaura
  • D'Ofrio, Claudio
  • De Lorenzi, Gabriela
  • Ferreira, Vanessa
  • Pinto-Carnide, Olinda
Item Type: Article
Título de Revista/Publicación: Molecular Biotechnology
Date: September 2012
ISSN: 1073-6085
Volume: 52
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biología Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 true-to-type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinhão cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based polymorphisms, 18 primers based on the LTR sequences of Tvv1, Gret1 and Vine-1 were used. In the analysis of 41 cultivars, 517 IRAP, REMAP, AFLP and SSAP fragments were obtained, 83% of which were polymorphic. For IRAP, only the Tvv1Fa primer amplified DNA fragments. In the REMAP analysis, the Tvv1Fa-Ms14 primer combination only produced polymorphic bands, and the Vine-1 primers produced mainly ISSR fragments. The highest number of polymorphic fragments was found for AFLP. Both AFLP and SSAP showed a greater capacity for identifying clones, resulting in 15 and 9 clones identified, respectively. Together, all of the techniques allowed for the identification of 54% of the studied clones, which is an important step in solving one of the challenges that viticulture currently faces.

More information

Item ID: 15079
DC Identifier: http://oa.upm.es/15079/
OAI Identifier: oai:oa.upm.es:15079
DOI: 10.1007/s12033-011-9470-y
Official URL: http://link.springer.com/article/10.1007/s12033-011-9470-y
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2013 14:29
Last Modified: 21 Apr 2016 15:10
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