Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones

Ortiz Marcide, Jesus Maria; Martin Clemente, Juan Pedro; Castro, Isaura; D'Ofrio, Claudio; De Lorenzi, Gabriela; Ferreira, Vanessa y Pinto-Carnide, Olinda (2012). Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones. "Molecular Biotechnology", v. 52 (n. 1); pp. 26-39. ISSN 1073-6085. https://doi.org/10.1007/s12033-011-9470-y.

Descripción

Título: Effectiveness of AFLPs and retrotransposon-based markers for the identification of portuguese grapevine cultivars and clones
Autor/es:
  • Ortiz Marcide, Jesus Maria
  • Martin Clemente, Juan Pedro
  • Castro, Isaura
  • D'Ofrio, Claudio
  • De Lorenzi, Gabriela
  • Ferreira, Vanessa
  • Pinto-Carnide, Olinda
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Biotechnology
Fecha: Septiembre 2012
Volumen: 52
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biología Vegetal [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Grapevine germplasm, including 38 of the main Portuguese cultivars and three foreign cultivars, Pinot Noir, Pinot Blanc and Chasselas, used as a reference, and 37 true-to-type clones from the Alvarinho, Arinto, Loureiro, Moscatel Galego Branco, Trajadura and Vinhão cultivars were studied using AFLP and three retrotransposon-based molecular techniques, IRAP, REMAP and SSAP. To study the retrotransposon-based polymorphisms, 18 primers based on the LTR sequences of Tvv1, Gret1 and Vine-1 were used. In the analysis of 41 cultivars, 517 IRAP, REMAP, AFLP and SSAP fragments were obtained, 83% of which were polymorphic. For IRAP, only the Tvv1Fa primer amplified DNA fragments. In the REMAP analysis, the Tvv1Fa-Ms14 primer combination only produced polymorphic bands, and the Vine-1 primers produced mainly ISSR fragments. The highest number of polymorphic fragments was found for AFLP. Both AFLP and SSAP showed a greater capacity for identifying clones, resulting in 15 and 9 clones identified, respectively. Together, all of the techniques allowed for the identification of 54% of the studied clones, which is an important step in solving one of the challenges that viticulture currently faces.

Más información

ID de Registro: 15079
Identificador DC: http://oa.upm.es/15079/
Identificador OAI: oai:oa.upm.es:15079
Identificador DOI: 10.1007/s12033-011-9470-y
URL Oficial: http://link.springer.com/article/10.1007/s12033-011-9470-y
Depositado por: Memoria Investigacion
Depositado el: 05 Jun 2013 14:29
Ultima Modificación: 21 Abr 2016 15:10
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