Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments

Amor Parrilla, Fermín and Christiaens, Olivier and Bengochea Budia, Paloma and Medina Velez, Maria Pilar and Rougé, Pierre and Viñuela Sandoval, Elisa and Smagghe, Guy (2012). Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments. "Pest Management Science", v. 68 (n. 12); pp.. ISSN 1526-4998.

Description

Title: Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments
Author/s:
  • Amor Parrilla, Fermín
  • Christiaens, Olivier
  • Bengochea Budia, Paloma
  • Medina Velez, Maria Pilar
  • Rougé, Pierre
  • Viñuela Sandoval, Elisa
  • Smagghe, Guy
Item Type: Article
Título de Revista/Publicación: Pest Management Science
Date: December 2012
ISSN: 1526-4998
Volume: 68
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Vegetal: Botánica y Protección Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

BACKGROUND: Knowledge of pesticide selectivity to natural enemies is necessary for a successful implementation of biological and chemical control methods in integrated pest management (IPM) programs. Diacylhydrazine (DAH)-based ecdysone agonists also known as molting-accelerating compounds (MACs) are considered a selective group of insecticides, and their compatibility with predatory Heteroptera, which are used as biological control agents, is known. However, their molecular mode of action has not been explored in beneficial insects such as Orius laevigatus (Fieber) (Hemiptera: Anthocoridae). RESULTS: In this project in vivo toxicity assays demonstrated that the DAH-based RH-5849, tebufenozide and methoxyfenozide have no toxic effect against O. laevigatus. The ligand-binding domain (LBD) of the ecdysone receptor (EcR) of O. laevigatus was sequenced and a homology protein model was constructed which confirmed a cavity structure with 12 ?-helixes, harboring the natural insect molting hormone 20-hydroxyecdysone. However, docking studies showed that a steric clash occurred for the DAH-based insecticides due to a restricted extent of the ligand-binding cavity of the EcR of O. laevigatus. CONCLUSIONS: The insect toxicity assays demonstrated that MACs are selective for O. laevigatus. The modeling/docking experiments are indications that these pesticides do not bind with the LBD-EcR of O. laevigatus and support that they show no biological effects in the predatory bug. These data help in explaining the compatible use of MACs together with predatory bugs in IPM programs. Keywords: Orius laevigatus, selectivity, diacylhydrazine insecticides, ecdysone receptor, homology modelling, docking studies.

More information

Item ID: 15254
DC Identifier: http://oa.upm.es/15254/
OAI Identifier: oai:oa.upm.es:15254
Official URL: http://onlinelibrary.wiley.com/doi/10.1002/ps.3353/abstract
Deposited by: Memoria Investigacion
Deposited on: 13 Jun 2013 13:38
Last Modified: 21 Apr 2016 15:18
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