Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments

Amor Parrilla, Fermín; Christiaens, Olivier; Bengochea Budia, Paloma; Medina Velez, Maria Pilar; Rougé, Pierre; Viñuela Sandoval, Elisa y Smagghe, Guy (2012). Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments. "Pest Management Science", v. 68 (n. 12); pp.. ISSN 1526-4998.

Descripción

Título: Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments
Autor/es:
  • Amor Parrilla, Fermín
  • Christiaens, Olivier
  • Bengochea Budia, Paloma
  • Medina Velez, Maria Pilar
  • Rougé, Pierre
  • Viñuela Sandoval, Elisa
  • Smagghe, Guy
Tipo de Documento: Artículo
Título de Revista/Publicación: Pest Management Science
Fecha: Diciembre 2012
Volumen: 68
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Producción Vegetal: Botánica y Protección Vegetal [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

BACKGROUND: Knowledge of pesticide selectivity to natural enemies is necessary for a successful implementation of biological and chemical control methods in integrated pest management (IPM) programs. Diacylhydrazine (DAH)-based ecdysone agonists also known as molting-accelerating compounds (MACs) are considered a selective group of insecticides, and their compatibility with predatory Heteroptera, which are used as biological control agents, is known. However, their molecular mode of action has not been explored in beneficial insects such as Orius laevigatus (Fieber) (Hemiptera: Anthocoridae). RESULTS: In this project in vivo toxicity assays demonstrated that the DAH-based RH-5849, tebufenozide and methoxyfenozide have no toxic effect against O. laevigatus. The ligand-binding domain (LBD) of the ecdysone receptor (EcR) of O. laevigatus was sequenced and a homology protein model was constructed which confirmed a cavity structure with 12 ?-helixes, harboring the natural insect molting hormone 20-hydroxyecdysone. However, docking studies showed that a steric clash occurred for the DAH-based insecticides due to a restricted extent of the ligand-binding cavity of the EcR of O. laevigatus. CONCLUSIONS: The insect toxicity assays demonstrated that MACs are selective for O. laevigatus. The modeling/docking experiments are indications that these pesticides do not bind with the LBD-EcR of O. laevigatus and support that they show no biological effects in the predatory bug. These data help in explaining the compatible use of MACs together with predatory bugs in IPM programs. Keywords: Orius laevigatus, selectivity, diacylhydrazine insecticides, ecdysone receptor, homology modelling, docking studies.

Más información

ID de Registro: 15254
Identificador DC: http://oa.upm.es/15254/
Identificador OAI: oai:oa.upm.es:15254
URL Oficial: http://onlinelibrary.wiley.com/doi/10.1002/ps.3353/abstract
Depositado por: Memoria Investigacion
Depositado el: 13 Jun 2013 13:38
Ultima Modificación: 21 Abr 2016 15:18
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