HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism

Oomen, Ronald J.f.j. and Benito Casado, Begoña and Sentenac, Hervé and Rodriguez Navarro, Alonso and Talon, Manuel and Very, Anne-Allienor and Domingo, Concha (2012). HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism. "Plant Journal", v. 71 (n. 5); pp. 750-762. ISSN 0960-7412.

Description

Title: HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism
Author/s:
  • Oomen, Ronald J.f.j.
  • Benito Casado, Begoña
  • Sentenac, Hervé
  • Rodriguez Navarro, Alonso
  • Talon, Manuel
  • Very, Anne-Allienor
  • Domingo, Concha
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: September 2012
ISSN: 0960-7412
Volume: 71
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (9MB) | Preview

Abstract

We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5¢ region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3¢ region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions.

More information

Item ID: 15257
DC Identifier: http://oa.upm.es/15257/
OAI Identifier: oai:oa.upm.es:15257
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.05031.x/abstract
Deposited by: Memoria Investigacion
Deposited on: 13 Jun 2013 17:08
Last Modified: 21 Apr 2016 15:18
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM