HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism

Oomen, Ronald J.f.j.; Benito Casado, Begoña; Sentenac, Hervé; Rodriguez Navarro, Alonso; Talon, Manuel; Very, Anne-Allienor y Domingo, Concha (2012). HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism. "Plant Journal", v. 71 (n. 5); pp. 750-762. ISSN 0960-7412.

Descripción

Título: HKT2;2/1, a K+-permeable transporter identified in a salt tolerant rice cultivar through surveys of natural genetic polymorphism
Autor/es:
  • Oomen, Ronald J.f.j.
  • Benito Casado, Begoña
  • Sentenac, Hervé
  • Rodriguez Navarro, Alonso
  • Talon, Manuel
  • Very, Anne-Allienor
  • Domingo, Concha
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Journal
Fecha: Septiembre 2012
Volumen: 71
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

We have investigated OsHKT2;1 natural variation in a collection of 49 cultivars with different levels of salt tolerance and geographical origins. The effect of identified polymorphism on OsHKT2;1 activity was analysed through heterologous expression of variants in Xenopus oocytes. OsHKT2;1 appeared to be a highly conserved protein with only five possible amino acid substitutions that have no substantial effect on functional properties. Our study, however, also identified a new HKT isoform, No-OsHKT2;2/1 in Nona Bokra, a highly salt-tolerant cultivar. No-OsHKT2;2/1 probably originated from a deletion in chromosome 6, producing a chimeric gene. Its 5¢ region corresponds to that of OsHKT2;2, whose full-length sequence is not present in Nipponbare but has been identified in Pokkali, a salt-tolerant rice cultivar. Its 3¢ region corresponds to that of OsHKT2;1. No-OsHKT2;2/1 is essentially expressed in roots and displays a significant level of expression at high Na+ concentrations, in contrast to OsHKT2;1. Expressed in Xenopus oocytes or in Saccharomyces cerevisiae, No-OsHKT2;2/1 exhibited a strong permeability to Na+ and K+, even at high external Na+ concentrations, like OsHKT2;2, and in contrast to OsHKT2;1. Our results suggest that No-OsHKT2;2/1 can contribute to Nona Bokra salt tolerance by enabling root K+ uptake under saline conditions.

Más información

ID de Registro: 15257
Identificador DC: http://oa.upm.es/15257/
Identificador OAI: oai:oa.upm.es:15257
URL Oficial: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2012.05031.x/abstract
Depositado por: Memoria Investigacion
Depositado el: 13 Jun 2013 17:08
Ultima Modificación: 21 Abr 2016 15:18
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