Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon

Giraldo Carbajo, Patricia; Rodriguez De Quijano Urquiaga, Marta; Vazquez Muñiz, Jose Francisco; Carrillo Becerril, Jose Maria y Benavente Barzana, M. Elena (2012). Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon. "Genome", v. 55 (n. 7); pp. 523-527. ISSN 0831-2796. https://doi.org/10.1139/g2012-039.

Descripción

Título: Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon
Autor/es:
  • Giraldo Carbajo, Patricia
  • Rodriguez De Quijano Urquiaga, Marta
  • Vazquez Muñiz, Jose Francisco
  • Carrillo Becerril, Jose Maria
  • Benavente Barzana, M. Elena
Tipo de Documento: Artículo
Título de Revista/Publicación: Genome
Fecha: Julio 2012
Volumen: 55
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Brachypodium distachyon (2n = 2x = 10) is a small annual grass species where the existence of three different cytotypes (10, 20 and 30 chromosomes) has long been regarded as a case of autopolyploid series, with x = 5. However, it has been demonstrated that the cytotypes assumed to be polyploids represent two separate Brachypodium species recently named as B. stacei (2n = 2x = 20) and B. hybridum (2n = 4x = 30). The aim of this study was to find a PCR-based alternative approach that could replace standard cytotyping methods (i. e., chromosome counting and flow cytometry) to characterize each of the three Brachypodium species. We have analyzed with four microsatellite (SSR) markers eighty-three Brachypodium distachyon-type lines from varied locations in Spain, including the Balearic and Canary Islands. Within this set of lines, 64, 4 and 15 had 10, 20 and 30 chromosomes, respectively. The surveyed markers produced cytotype-specific SSR profiles. So, a single amplification product was generated in the diploid samples, with non-overlapping allelic ranges between the 2n = 10 and 2n = 20 cytotypes, whereas two bands, one in the size range of each of the diploid cytotypes, were amplified in the 2n = 30 lines. Furthermore, the remarkable size difference obtained with the SSR ALB165 allowed the identification of the Brachypodium species by simple agarose gel electrophoresis.

Más información

ID de Registro: 15435
Identificador DC: http://oa.upm.es/15435/
Identificador OAI: oai:oa.upm.es:15435
Identificador DOI: 10.1139/g2012-039
URL Oficial: http://www.nrcresearchpress.com/doi/abs/10.1139/g2012-039
Depositado por: Memoria Investigacion
Depositado el: 17 Jun 2013 13:54
Ultima Modificación: 21 Abr 2016 15:29
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