Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon

Giraldo Carbajo, Patricia and Rodriguez De Quijano Urquiaga, Marta and Vazquez Muñiz, Jose Francisco and Carrillo Becerril, Jose Maria and Benavente Barzana, M. Elena (2012). Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon. "Genome", v. 55 (n. 7); pp. 523-527. ISSN 0831-2796. https://doi.org/10.1139/g2012-039.

Description

Title: Validation of microsatellite markers for cytotype discrimination in the model grass Brachypodium distachyon
Author/s:
  • Giraldo Carbajo, Patricia
  • Rodriguez De Quijano Urquiaga, Marta
  • Vazquez Muñiz, Jose Francisco
  • Carrillo Becerril, Jose Maria
  • Benavente Barzana, M. Elena
Item Type: Article
Título de Revista/Publicación: Genome
Date: July 2012
ISSN: 0831-2796
Volume: 55
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Brachypodium distachyon (2n = 2x = 10) is a small annual grass species where the existence of three different cytotypes (10, 20 and 30 chromosomes) has long been regarded as a case of autopolyploid series, with x = 5. However, it has been demonstrated that the cytotypes assumed to be polyploids represent two separate Brachypodium species recently named as B. stacei (2n = 2x = 20) and B. hybridum (2n = 4x = 30). The aim of this study was to find a PCR-based alternative approach that could replace standard cytotyping methods (i. e., chromosome counting and flow cytometry) to characterize each of the three Brachypodium species. We have analyzed with four microsatellite (SSR) markers eighty-three Brachypodium distachyon-type lines from varied locations in Spain, including the Balearic and Canary Islands. Within this set of lines, 64, 4 and 15 had 10, 20 and 30 chromosomes, respectively. The surveyed markers produced cytotype-specific SSR profiles. So, a single amplification product was generated in the diploid samples, with non-overlapping allelic ranges between the 2n = 10 and 2n = 20 cytotypes, whereas two bands, one in the size range of each of the diploid cytotypes, were amplified in the 2n = 30 lines. Furthermore, the remarkable size difference obtained with the SSR ALB165 allowed the identification of the Brachypodium species by simple agarose gel electrophoresis.

More information

Item ID: 15435
DC Identifier: http://oa.upm.es/15435/
OAI Identifier: oai:oa.upm.es:15435
DOI: 10.1139/g2012-039
Official URL: http://www.nrcresearchpress.com/doi/abs/10.1139/g2012-039
Deposited by: Memoria Investigacion
Deposited on: 17 Jun 2013 13:54
Last Modified: 21 Apr 2016 15:29
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