Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media

Benito Saez, Santiago and Palomero Rodriguez, Felipe and Morata Barrado, Antonio and Calderon Fernandez, Fernando and Palmero Llamas, Daniel and Suarez Lepe, Jose Antonio (2012). Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media. "African Journal of Microbiology Research", v. 34 (n. 6); pp. 6348-6357. ISSN 1996-0808.

Description

Title: Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media
Author/s:
  • Benito Saez, Santiago
  • Palomero Rodriguez, Felipe
  • Morata Barrado, Antonio
  • Calderon Fernandez, Fernando
  • Palmero Llamas, Daniel
  • Suarez Lepe, Jose Antonio
Item Type: Article
Título de Revista/Publicación: African Journal of Microbiology Research
Date: 2012
ISSN: 1996-0808
Volume: 34
Subjects:
Faculty: E.U.I.T. Agrícolas (UPM)
Department: Ciencia y Tecnología Aplicadas a la Ingeniería Técnica Agrícola [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (354kB) | Preview

Abstract

The purpose of this work was to compare and optimise different selective and differential media to aid in isolating spoilage yeasts belonging to the Brettanomyces/Dekkera genera. Growth media containing selective and differential factors were employed. These were inoculated with strains of yeast representing Spanish oenological microbiota. Lastly, some of these isolation media were successfully applied in 24 types of wine with a high ethylphenol content, all of which were from the Haro Oenological Station (La Rioja, Spain). p-coumaric acid was determined using High performance liquid chromatography-photodiode-array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS); 4-ethylphenol by using Solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GC-MS); and the rest of the analysis was carried out using official OIV methodology. Actidione is the most effective selective factor for isolating Brettanomyces/Dekkera yeast genera. Other secondary selective factors (selective carbon sources, sorbic acid and ethanol as a microbicide agent) may be used successfully to eliminate potential false positivities; however, they slow growth and delay the time to obtain results.

More information

Item ID: 15560
DC Identifier: http://oa.upm.es/15560/
OAI Identifier: oai:oa.upm.es:15560
Deposited by: Memoria Investigacion
Deposited on: 15 Jul 2013 09:20
Last Modified: 21 Apr 2016 15:41
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM