Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media

Benito Saez, Santiago; Palomero Rodriguez, Felipe; Morata Barrado, Antonio; Calderon Fernandez, Fernando; Palmero Llamas, Daniel y Suarez Lepe, Jose Antonio (2012). Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media. "African Journal of Microbiology Research", v. 34 (n. 6); pp. 6348-6357. ISSN 1996-0808.

Descripción

Título: Identifying yeats belonging to the Brettanomyces/Dekkera genera through the use of selective-differential media
Autor/es:
  • Benito Saez, Santiago
  • Palomero Rodriguez, Felipe
  • Morata Barrado, Antonio
  • Calderon Fernandez, Fernando
  • Palmero Llamas, Daniel
  • Suarez Lepe, Jose Antonio
Tipo de Documento: Artículo
Título de Revista/Publicación: African Journal of Microbiology Research
Fecha: 2012
Volumen: 34
Materias:
Escuela: E.U.I.T. Agrícolas (UPM)
Departamento: Ciencia y Tecnología Aplicadas a la Ingeniería Técnica Agrícola [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The purpose of this work was to compare and optimise different selective and differential media to aid in isolating spoilage yeasts belonging to the Brettanomyces/Dekkera genera. Growth media containing selective and differential factors were employed. These were inoculated with strains of yeast representing Spanish oenological microbiota. Lastly, some of these isolation media were successfully applied in 24 types of wine with a high ethylphenol content, all of which were from the Haro Oenological Station (La Rioja, Spain). p-coumaric acid was determined using High performance liquid chromatography-photodiode-array detection-electrospray ionization mass spectrometry (HPLC-DAD-ESI/MS); 4-ethylphenol by using Solid phase micro extraction-gas chromatography-mass spectrometry (SPME-GC-MS); and the rest of the analysis was carried out using official OIV methodology. Actidione is the most effective selective factor for isolating Brettanomyces/Dekkera yeast genera. Other secondary selective factors (selective carbon sources, sorbic acid and ethanol as a microbicide agent) may be used successfully to eliminate potential false positivities; however, they slow growth and delay the time to obtain results.

Más información

ID de Registro: 15560
Identificador DC: http://oa.upm.es/15560/
Identificador OAI: oai:oa.upm.es:15560
Depositado por: Memoria Investigacion
Depositado el: 15 Jul 2013 09:20
Ultima Modificación: 21 Abr 2016 15:41
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