Embryogenic suspensions of adult cork oak: the first step towards mass propagation.

Jiménez García, Jesús; López Vela, Dolores; Ruiz Galea, Mar; Celestino, Cristina; Toribio, Mariano y Alegre, Jesús (2013). Embryogenic suspensions of adult cork oak: the first step towards mass propagation.. "Trees-Structure And Function", v. 27 (n. 1); pp. 13-23. ISSN 0931-1890. https://doi.org/10.1007/s00468-012-0763-y.

Descripción

Título: Embryogenic suspensions of adult cork oak: the first step towards mass propagation.
Autor/es:
  • Jiménez García, Jesús
  • López Vela, Dolores
  • Ruiz Galea, Mar
  • Celestino, Cristina
  • Toribio, Mariano
  • Alegre, Jesús
Tipo de Documento: Artículo
Título de Revista/Publicación: Trees-Structure And Function
Fecha: Febrero 2013
Volumen: 27
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (8MB) | Vista Previa

Resumen

Abstract Protocols have been established to clone adult cork oak trees by somatic embryogenesis using semisolid medium. However, for economically viable mass propagation, embryogenic cultures in liquid medium need to be developed. In this study, suspension cultures were initiated from embryo clusters obtained by secondary embryogenesis on a gelled medium lacking plant growth regulators. After 6 days of culture, these embryo clusters generated high cell density suspensions that also contained small organized structures (embryos and embryogenic clumps). As the culture duration increased, tissue necrosis and fewer embryogenic structures were observed and the establishment of suspension cultures failed. An alternative method was found adequate for initiation of embryogenic suspensions: embryo clusters from gelled medium were briefly shaken in liquid medium and detached cells and embryogenic masses of 41?800 lm were used as inoculum. Maintenance of embryogenic suspensions was achieved using a low-density inoculum (43 mg l-1) by subculturing four embryogenic clumps of 0.8?1.2 mm per 70 ml of medium. Proliferation ability was maintained for almost 1 year through ten consecutive subcultures. The initiation and maintenance protocols first developed for a single genotype were effective when tested on 11 cork oak genotypes.

Más información

ID de Registro: 15562
Identificador DC: http://oa.upm.es/15562/
Identificador OAI: oai:oa.upm.es:15562
Identificador DOI: 10.1007/s00468-012-0763-y
URL Oficial: http://link.springer.com/article/10.1007/s00468-012-0763-y
Depositado por: Memoria Investigacion
Depositado el: 05 Jun 2013 15:39
Ultima Modificación: 21 Abr 2016 15:43
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM