Embryogenic suspensions of adult cork oak: the first step towards mass propagation.

Jiménez García, Jesús and López Vela, Dolores and Ruiz Galea, Mar and Celestino, Cristina and Toribio, Mariano and Alegre, Jesús (2013). Embryogenic suspensions of adult cork oak: the first step towards mass propagation.. "Trees-Structure And Function", v. 27 (n. 1); pp. 13-23. ISSN 0931-1890. https://doi.org/10.1007/s00468-012-0763-y.

Description

Title: Embryogenic suspensions of adult cork oak: the first step towards mass propagation.
Author/s:
  • Jiménez García, Jesús
  • López Vela, Dolores
  • Ruiz Galea, Mar
  • Celestino, Cristina
  • Toribio, Mariano
  • Alegre, Jesús
Item Type: Article
Título de Revista/Publicación: Trees-Structure And Function
Date: February 2013
ISSN: 0931-1890
Volume: 27
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Abstract Protocols have been established to clone adult cork oak trees by somatic embryogenesis using semisolid medium. However, for economically viable mass propagation, embryogenic cultures in liquid medium need to be developed. In this study, suspension cultures were initiated from embryo clusters obtained by secondary embryogenesis on a gelled medium lacking plant growth regulators. After 6 days of culture, these embryo clusters generated high cell density suspensions that also contained small organized structures (embryos and embryogenic clumps). As the culture duration increased, tissue necrosis and fewer embryogenic structures were observed and the establishment of suspension cultures failed. An alternative method was found adequate for initiation of embryogenic suspensions: embryo clusters from gelled medium were briefly shaken in liquid medium and detached cells and embryogenic masses of 41?800 lm were used as inoculum. Maintenance of embryogenic suspensions was achieved using a low-density inoculum (43 mg l-1) by subculturing four embryogenic clumps of 0.8?1.2 mm per 70 ml of medium. Proliferation ability was maintained for almost 1 year through ten consecutive subcultures. The initiation and maintenance protocols first developed for a single genotype were effective when tested on 11 cork oak genotypes.

More information

Item ID: 15562
DC Identifier: http://oa.upm.es/15562/
OAI Identifier: oai:oa.upm.es:15562
DOI: 10.1007/s00468-012-0763-y
Official URL: http://link.springer.com/article/10.1007/s00468-012-0763-y
Deposited by: Memoria Investigacion
Deposited on: 05 Jun 2013 15:39
Last Modified: 21 Apr 2016 15:43
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