Plant regeneration from an endangered valuable cork oak tree by somatic embryogenesis.

Hernández, Inmaculada; Celestino, Cristina; López Vela, Dolores; Carneros, Elena; Jiménez García, Jesús; Alegre, Jesús; Gil Sanchez, Luis y Toribio, Mariano (2008). Plant regeneration from an endangered valuable cork oak tree by somatic embryogenesis.. En: "Sin especificar. pp. 205-211.

Descripción

Título: Plant regeneration from an endangered valuable cork oak tree by somatic embryogenesis.
Autor/es:
  • Hernández, Inmaculada
  • Celestino, Cristina
  • López Vela, Dolores
  • Carneros, Elena
  • Jiménez García, Jesús
  • Alegre, Jesús
  • Gil Sanchez, Luis
  • Toribio, Mariano
Tipo de Documento: Ponencia en Congreso o Jornada (Otro)
Título del Libro: SUBERWOOD. New challenges for the integration of cork oak forest and products
Título de Revista/Publicación: Suberwood 2005: New challenges for integration of cork oak forest and products.
Fecha: 2008
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Among the several applications of in vitro tissue culture techniques, the conservation of plant germplasm is one of the most widely used. The cork oak is one of the principal tree species in the Western Mediterranean región. Within this área, the Balearic Islands are considered to be a glacial refuge, and therefore a reservoir of genetic resources. A singular tree has been found in the small Minorca Island population. The haplotype of this tree is of Tyrrhenian origin, showing a past link between Minorca and Sardinia. Moreover, this tree do not bear a deletion within an ITS from ribosimic nuclear DNA, which is fairly common in many populations of this species, and indicates that ir may be the descendant of a very ancient population. This tree is currently in a precarious condition, and it has not produced acorns in the last years. Hence there is a clear need of vegetative propagation to conserve this genotype. We have previously developed methods to clone adult cork oak tres by somatic embryogenesis, and therefore the aim of the present work was to clone this singular tree. There braches from the corwn were collected in November 2004, and methods previously described were carried out. By February 2005 somatic embryogenesis was obtained from leaves of the tree with percentages on induction ranging from 17 to 54% depending on the branch, which may show a novel source of variation that requires further study. Spontaneously matured somatic embryos germinated at 46% in average, and the first somatic seedlings from the Alfavaret's cork oak tree were obtained. Therefore, this study shows one of the most relevant applications of somatic embryogenesis: the plant regeneration of valuable genotypes for the in situ and ex situ conservation of forest genetic resources.

Más información

ID de Registro: 15705
Identificador DC: http://oa.upm.es/15705/
Identificador OAI: oai:oa.upm.es:15705
Depositado por: Memoria Investigacion
Depositado el: 20 Mar 2014 13:28
Ultima Modificación: 21 Abr 2016 15:58
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