Plant regeneration from an endangered valuable cork oak tree by somatic embryogenesis.

Hernández, Inmaculada and Celestino, Cristina and López Vela, Dolores and Carneros, Elena and Jiménez García, Jesús and Alegre, Jesús and Gil Sanchez, Luis Alfonso and Toribio, Mariano (2008). Plant regeneration from an endangered valuable cork oak tree by somatic embryogenesis.. In: "Unspecified. pp. 205-211.

Description

Title: Plant regeneration from an endangered valuable cork oak tree by somatic embryogenesis.
Author/s:
  • Hernández, Inmaculada
  • Celestino, Cristina
  • López Vela, Dolores
  • Carneros, Elena
  • Jiménez García, Jesús
  • Alegre, Jesús
  • Gil Sanchez, Luis Alfonso
  • Toribio, Mariano
Item Type: Presentation at Congress or Conference (Other)
Title of Book: SUBERWOOD. New challenges for the integration of cork oak forest and products
Título de Revista/Publicación: Suberwood 2005: New challenges for integration of cork oak forest and products.
Date: 2008
ISSN: 978-84-96826-47-2
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Among the several applications of in vitro tissue culture techniques, the conservation of plant germplasm is one of the most widely used. The cork oak is one of the principal tree species in the Western Mediterranean región. Within this área, the Balearic Islands are considered to be a glacial refuge, and therefore a reservoir of genetic resources. A singular tree has been found in the small Minorca Island population. The haplotype of this tree is of Tyrrhenian origin, showing a past link between Minorca and Sardinia. Moreover, this tree do not bear a deletion within an ITS from ribosimic nuclear DNA, which is fairly common in many populations of this species, and indicates that ir may be the descendant of a very ancient population. This tree is currently in a precarious condition, and it has not produced acorns in the last years. Hence there is a clear need of vegetative propagation to conserve this genotype. We have previously developed methods to clone adult cork oak tres by somatic embryogenesis, and therefore the aim of the present work was to clone this singular tree. There braches from the corwn were collected in November 2004, and methods previously described were carried out. By February 2005 somatic embryogenesis was obtained from leaves of the tree with percentages on induction ranging from 17 to 54% depending on the branch, which may show a novel source of variation that requires further study. Spontaneously matured somatic embryos germinated at 46% in average, and the first somatic seedlings from the Alfavaret's cork oak tree were obtained. Therefore, this study shows one of the most relevant applications of somatic embryogenesis: the plant regeneration of valuable genotypes for the in situ and ex situ conservation of forest genetic resources.

More information

Item ID: 15705
DC Identifier: http://oa.upm.es/15705/
OAI Identifier: oai:oa.upm.es:15705
Deposited by: Memoria Investigacion
Deposited on: 20 Mar 2014 13:28
Last Modified: 10 Jan 2019 13:01
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