Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species

Aguinagalde Madariaga, M. Itziar Josefina; Maselli, Silvia y Perez Garcia, Felix (2012). Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species. "Seed Science And Technology", v. 40 (n. 3); pp. 365-373. ISSN 0251-0952.

Descripción

Título: Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species
Autor/es:
  • Aguinagalde Madariaga, M. Itziar Josefina
  • Maselli, Silvia
  • Perez Garcia, Felix
Tipo de Documento: Artículo
Título de Revista/Publicación: Seed Science And Technology
Fecha: Octubre 2012
Volumen: 40
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biología Vegetal [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img] PDF (Document Portable Format) - Acceso permitido solamente a usuarios en el campus de la UPM - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (48kB)

Resumen

The main objective of this study was to determine if isozyme systems can be used as markers of genetic deterioration in Brassicaceae seed accessions under different storage conditions. Seed samples of Brassica oleracea, Cardaria draba, Erysimum cheiri, Iberis sempervirens and Rapistrum rugosum were stored for periods of 9 to 30 years at -10°C and 3-4% seed moisture content (long-term or LT conditions) and at 5°C and uncontrolled relative humidity (RH) (short-term or ST conditions). Starch Gel Electrophoresis (SGE) was used to analyse six enzyme systems oriented to determine the genetic deterioration of the accessions studied. The results obtained show that long-term storage conditions (LT) were extremely effective in maintaining the viability of seeds of the five Brassicaceae species studied. The final germination percentages reached by seeds from LT samples ranged from 75 to 100%, while the germination percentages of ST samples (except for B. oleracea) were very low (from 0 to 10%). Similar conclusions were obtained studying the integrity of electrophoretic bands for several isozymes. Two enzyme systems were of special interest: malate dehydrogenase and alcohol dehydrogenase.

Más información

ID de Registro: 15768
Identificador DC: http://oa.upm.es/15768/
Identificador OAI: oai:oa.upm.es:15768
URL Oficial: http://www.ingentaconnect.com/content/ista/sst/2012/00000040/00000003/art00008
Depositado por: Memoria Investigacion
Depositado el: 14 Jun 2013 12:41
Ultima Modificación: 22 Sep 2014 11:09
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM