Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species

Aguinagalde Madariaga, M. Itziar Josefina and Maselli, Silvia and Perez Garcia, Felix (2012). Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species. "Seed Science And Technology", v. 40 (n. 3); pp. 365-373. ISSN 0251-0952.

Description

Title: Isozyme systems as markers of genetic deterioration in stored seeds of Brassicaceae species
Author/s:
  • Aguinagalde Madariaga, M. Itziar Josefina
  • Maselli, Silvia
  • Perez Garcia, Felix
Item Type: Article
Título de Revista/Publicación: Seed Science And Technology
Date: October 2012
ISSN: 0251-0952
Volume: 40
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biología Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The main objective of this study was to determine if isozyme systems can be used as markers of genetic deterioration in Brassicaceae seed accessions under different storage conditions. Seed samples of Brassica oleracea, Cardaria draba, Erysimum cheiri, Iberis sempervirens and Rapistrum rugosum were stored for periods of 9 to 30 years at -10°C and 3-4% seed moisture content (long-term or LT conditions) and at 5°C and uncontrolled relative humidity (RH) (short-term or ST conditions). Starch Gel Electrophoresis (SGE) was used to analyse six enzyme systems oriented to determine the genetic deterioration of the accessions studied. The results obtained show that long-term storage conditions (LT) were extremely effective in maintaining the viability of seeds of the five Brassicaceae species studied. The final germination percentages reached by seeds from LT samples ranged from 75 to 100%, while the germination percentages of ST samples (except for B. oleracea) were very low (from 0 to 10%). Similar conclusions were obtained studying the integrity of electrophoretic bands for several isozymes. Two enzyme systems were of special interest: malate dehydrogenase and alcohol dehydrogenase.

More information

Item ID: 15768
DC Identifier: http://oa.upm.es/15768/
OAI Identifier: oai:oa.upm.es:15768
Official URL: http://www.ingentaconnect.com/content/ista/sst/2012/00000040/00000003/art00008
Deposited by: Memoria Investigacion
Deposited on: 14 Jun 2013 12:41
Last Modified: 22 Sep 2014 11:09
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