Monte-Carlo dosimetry on a realistic cell monolayer geometry exposed to alpha particles

Barberet, Philippe and Vianna, François and Karamitros, M. and Brun, T. and Gordillo Garcia, Nuria and Moretto, Philippe and Incerti, Sébastien and Seznec, H. (2012). Monte-Carlo dosimetry on a realistic cell monolayer geometry exposed to alpha particles. "Physics in Medicine and Biology", v. 57 (n. 8); pp. 2189-2207. ISSN 0031-9155. https://doi.org/10.1088/0031-9155/57/8/2189.

Description

Title: Monte-Carlo dosimetry on a realistic cell monolayer geometry exposed to alpha particles
Author/s:
  • Barberet, Philippe
  • Vianna, François
  • Karamitros, M.
  • Brun, T.
  • Gordillo Garcia, Nuria
  • Moretto, Philippe
  • Incerti, Sébastien
  • Seznec, H.
Item Type: Article
Título de Revista/Publicación: Physics in Medicine and Biology
Date: April 2012
ISSN: 0031-9155
Volume: 57
Subjects:
Faculty: E.T.S.I. Industriales (UPM)
Department: Ingeniería Nuclear [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The energy and specific energy absorbed in the main cell compartments (nucleus and cytoplasm) in typical radiobiology experiments are usually estimated by calculations as they are not accessible for a direct measurement. In most of the work, the cell geometry is modelled using the combination of simple mathematical volumes. We propose a method based on high resolution confocal imaging and ion beam analysis (IBA) in order to import realistic cell nuclei geometries in Monte-Carlo simulations and thus take into account the variety of different geometries encountered in a typical cell population. Seventy-six cell nuclei have been imaged using confocal microscopy and their chemical composition has been measured using IBA. A cellular phantom was created from these data using the ImageJ image analysis software and imported in the Geant4 Monte-Carlo simulation toolkit. Total energy and specific energy distributions in the 76 cell nuclei have been calculated for two types of irradiation protocols: a 3 MeV alpha particle microbeam used for targeted irradiation and a 239Pu alpha source used for large angle random irradiation. Qualitative images of the energy deposited along the particle tracks have been produced and show good agreement with images of DNA double strand break signalling proteins obtained experimentally. The methodology presented in this paper provides microdosimetric quantities calculated from realistic cellular volumes. It is based on open-source oriented software that is publicly available.

More information

Item ID: 15955
DC Identifier: http://oa.upm.es/15955/
OAI Identifier: oai:oa.upm.es:15955
DOI: 10.1088/0031-9155/57/8/2189
Official URL: http://iopscience.iop.org/0031-9155/57/8/2189/
Deposited by: Memoria Investigacion
Deposited on: 17 Dec 2013 19:30
Last Modified: 21 Apr 2016 16:16
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