Luengo Oroz, Miguel Ángel and Rubio Guivernau, José Luis and Faure, Emmanuel and Savy, Thierry and Duloquin, Louise and Olivier, Nicolas and Pastor Escuredo, David and Ledesma Carbayo, María Jesús and Debarre, Delphine and Bourgine, Paul and Beaurepaire, Emmanuel and Peyrieras, Nadine and Santos Lleo, Andres de
Methodology for reconstructing early zebrafish development from in vivo multiphoton microscopy.
"IEEE Transactions on Image Processing", v. 21
Investigating cell dynamics during early zebrafish embryogenesis requires specific image acquisition and analysis strategies. Multiharmonic microscopy, i.e., second- and third-harmonic generations, allows imaging cell divisions and cell membranes in unstained zebrafish embryos from 1- to 1000-cell stage. This paper presents the design and implementation of a dedicated image processing pipeline (tracking and segmentation) for the reconstruction of cell dynamics during these developmental stages. This methodology allows the reconstruction of the cell lineage tree including division timings, spatial coordinates, and cell shape until the 1000-cell stage with minute temporal accuracy and micrometer spatial resolution. Data analysis of the digital embryos provides an extensive quantitative description of early zebrafish embryogenesis.