Palacín Gómez, Aranzazu and Gomez Casado, Cristina and Tordesillas Villuendas, Leticia and Sánchez-Monge Laguna de Rins, Rosa and Díaz Perales, Araceli and Rivas, Luis and Aguirre, Jacobo and Bartra, Joan and Blanco, Carlos and Carrillo, Teresa and Cuesta-Herranz, Javier and Cumplido Bonny, Jose and Flores, Enrique and García-Álvarez Eire, Mar and García-Núñez, Ignacio and Fernández, Francisco and Gamboa, P.M. and Muñoz, Rosa and Torres, María and Varela Losada, Susana and Villalba, M. and Vega, Francisco and Parro, Victor and Blanca, Miguel and Salcedo Duran, Gabriel
The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray..
"Plos One", v. 7
Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited mayor que50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.