Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.

Gómez, Enrique; Díaz Perales, Araceli; Tordesillas Villuendas, Leticia; Doña, Inmaculada; Torres, María; Blázquez, Ana; Gomez, Francisca; Blanca, Miguel y Mayorga, Cristobalina (2012). Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.. "Annals of Allergy Asthma & Immunology", v. 109 (n. 1); pp. 52-58. ISSN 1081-1206. https://doi.org/10.1016/j.anai.2012.05.017.

Descripción

Título: Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.
Autor/es:
  • Gómez, Enrique
  • Díaz Perales, Araceli
  • Tordesillas Villuendas, Leticia
  • Doña, Inmaculada
  • Torres, María
  • Blázquez, Ana
  • Gomez, Francisca
  • Blanca, Miguel
  • Mayorga, Cristobalina
Tipo de Documento: Artículo
Título de Revista/Publicación: Annals of Allergy Asthma & Immunology
Fecha: Julio 2012
Volumen: 109
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

BACKGROUND: Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known. OBJECTIVE: To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients. METHODS: Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 ?g/mL) by flow cytometry and cytokine production. RESULTS: Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls. CONCLUSION: Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls.

Más información

ID de Registro: 16235
Identificador DC: http://oa.upm.es/16235/
Identificador OAI: oai:oa.upm.es:16235
Identificador DOI: 10.1016/j.anai.2012.05.017
URL Oficial: http://www.annallergy.org/article/S1081-1206(12)00391-2/abstract
Depositado por: Memoria Investigacion
Depositado el: 05 Jul 2013 14:42
Ultima Modificación: 22 Sep 2014 11:11
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