Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.

Gómez, Enrique and Díaz Perales, Araceli and Tordesillas Villuendas, Leticia and Doña, Inmaculada and Torres, María and Blázquez, Ana and Gomez, Francisca and Blanca, Miguel and Mayorga, Cristobalina (2012). Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.. "Annals of Allergy Asthma & Immunology", v. 109 (n. 1); pp. 52-58. ISSN 1081-1206. https://doi.org/10.1016/j.anai.2012.05.017.

Description

Title: Effect of Pru p 3 on dendritic cell maturation and T-lymphocyte proliferation in peach allergic patients.
Author/s:
  • Gómez, Enrique
  • Díaz Perales, Araceli
  • Tordesillas Villuendas, Leticia
  • Doña, Inmaculada
  • Torres, María
  • Blázquez, Ana
  • Gomez, Francisca
  • Blanca, Miguel
  • Mayorga, Cristobalina
Item Type: Article
Título de Revista/Publicación: Annals of Allergy Asthma & Immunology
Date: July 2012
ISSN: 1081-1206
Volume: 109
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

BACKGROUND: Pru p 3 is the major peach allergen and the most frequent cause of food allergy in adults in the Mediterranean area. Although its allergenicity is well characterized, its ability to generate a T-cell response is not completely known. OBJECTIVE: To investigate the influence of Pru p 3 allergen on dendritic cell (DC) maturation and specific T-cell response (T(H)1/T(H)2) in peach allergic patients. METHODS: Peach allergic patients (n = 11) and tolerant controls (n = 14) were included in the study. Monocyte-derived DC maturation after incubation with Pru p 3 was evaluated by the increase of maturational markers (CD80, CD86, and CD83) by flow cytometry. Lymphocyte proliferation was evaluated by coculturing monocyte-derived DCs and 5,6-carboxyfluorescein diacetate N-succinimidyl ester-stained lymphocytes with different concentrations of Pru p 3 (25, 10, and 1 ?g/mL) by flow cytometry and cytokine production. RESULTS: Pru p 3 induced a significant increase in the CD80, CD86, and CD83 expression on stimulated DCs from patients compared with controls. The lymphocyte proliferative response after Pru p 3 stimulation was also significantly higher along with an increase in interleukin 8 in patients compared with tolerant controls. CONCLUSION: Pru p 3 allergen induces changes in DC maturational status mainly in peach allergic patients. An increase in lymphocyte proliferative response accompanied with a different cytokine pattern was also observed compared with healthy controls.

More information

Item ID: 16235
DC Identifier: http://oa.upm.es/16235/
OAI Identifier: oai:oa.upm.es:16235
DOI: 10.1016/j.anai.2012.05.017
Official URL: http://www.annallergy.org/article/S1081-1206(12)00391-2/abstract
Deposited by: Memoria Investigacion
Deposited on: 05 Jul 2013 14:42
Last Modified: 22 Sep 2014 11:11
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