Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillars

Ortega, Francisco J. and Bañuls, María José and Sanza Gutiérrez, Francisco Javier and Casquel del Campo, Rafael and Laguna Heras, Maria Fe and Holgado Bolaños, Miguel and López-Romero Moraleda, David and Angulo Barrios, Carlos and Maquieira, A. and Puchades, Rosa (2012). Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillars. "Biosensors", v. 2 (n. 3); pp. 291-304. ISSN 2079-6374. https://doi.org/10.3390/bios2030291.

Description

Title: Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillars
Author/s:
  • Ortega, Francisco J.
  • Bañuls, María José
  • Sanza Gutiérrez, Francisco Javier
  • Casquel del Campo, Rafael
  • Laguna Heras, Maria Fe
  • Holgado Bolaños, Miguel
  • López-Romero Moraleda, David
  • Angulo Barrios, Carlos
  • Maquieira, A.
  • Puchades, Rosa
Item Type: Article
Título de Revista/Publicación: Biosensors
Date: September 2012
ISSN: 2079-6374
Volume: 2
Subjects:
Freetext Keywords: micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination
Faculty: E.T.S.I. Telecomunicación (UPM)
Department: Ingeniería Electrónica
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).

More information

Item ID: 16735
DC Identifier: http://oa.upm.es/16735/
OAI Identifier: oai:oa.upm.es:16735
DOI: 10.3390/bios2030291
Official URL: http://www.mdpi.com/2079-6374/2/3/291
Deposited by: Memoria Investigacion
Deposited on: 07 Aug 2013 15:42
Last Modified: 28 Jun 2016 07:01
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