Disruption of abscisic acid signaling constitutively activates Arabidopsis resistance to the necrotrophic fungus Plectosphaerella cucumerina.

Sánchez Vallet, Andrea and López, Gemma and Ramos, Brisa and Delgado Cerezo, María Magdalena and Riviere, Marie Pierre and Llorente, Francisco and Fernández, Paula Virginia and Miedes Vicente, Eva and Estevez, José Manuel and Grant, Murray and Molina Fernández, Antonio (2012). Disruption of abscisic acid signaling constitutively activates Arabidopsis resistance to the necrotrophic fungus Plectosphaerella cucumerina.. "Plant Physiology", v. 160 ; pp. 2109-2124. ISSN 0032-0889. https://doi.org/10.1104/pp.112.200154.

Description

Title: Disruption of abscisic acid signaling constitutively activates Arabidopsis resistance to the necrotrophic fungus Plectosphaerella cucumerina.
Author/s:
  • Sánchez Vallet, Andrea
  • López, Gemma
  • Ramos, Brisa
  • Delgado Cerezo, María Magdalena
  • Riviere, Marie Pierre
  • Llorente, Francisco
  • Fernández, Paula Virginia
  • Miedes Vicente, Eva
  • Estevez, José Manuel
  • Grant, Murray
  • Molina Fernández, Antonio
Item Type: Article
Título de Revista/Publicación: Plant Physiology
Date: December 2012
ISSN: 0032-0889
Volume: 160
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Plant resistance to necrotrophic fungi is regulated by a complex set of signaling pathways that includes those mediated by the hormones salicylic acid (SA), ethylene (ET), jasmonic acid (JA), and abscisic acid (ABA). The role of ABA in plant resistance remains controversial, as positive and negative regulatory functions have been described depending on the plant-pathogen interaction analyzed. Here, we show that ABA signaling negatively regulates Arabidopsis (Arabidopsis thaliana) resistance to the necrotrophic fungus Plectosphaerella cucumerina. Arabidopsis plants impaired in ABA biosynthesis, such as the aba1-6 mutant, or in ABA signaling, like the quadruple pyr/pyl mutant (pyr1pyl1pyl2pyl4), were more resistant to P. cucumerina than wild-type plants. In contrast, the hab1-1abi1-2abi2-2 mutant impaired in three phosphatases that negatively regulate ABA signaling displayed an enhanced susceptibility phenotype to this fungus. Comparative transcriptomic analyses of aba1-6 and wild-type plants revealed that the ABA pathway negatively regulates defense genes, many of which are controlled by the SA, JA, or ET pathway. In line with these data, we found that aba1-6 resistance to P. cucumerina was partially compromised when the SA, JA, or ET pathway was disrupted in this mutant. Additionally, in the aba1-6 plants, some genes encoding cell wall-related proteins were misregulated. Fourier transform infrared spectroscopy and biochemical analyses of cell walls from aba1-6 and wild-type plants revealed significant differences in their Fourier transform infrared spectratypes and uronic acid and cellulose contents. All these data suggest that ABA signaling has a complex function in Arabidopsis basal resistance, negatively regulating SA/JA/ET-mediated resistance to necrotrophic fungi.

More information

Item ID: 16746
DC Identifier: http://oa.upm.es/16746/
OAI Identifier: oai:oa.upm.es:16746
DOI: 10.1104/pp.112.200154
Official URL: http://www.plantphysiol.org/content/160/4/2109.abstract
Deposited by: Memoria Investigacion
Deposited on: 06 Sep 2013 17:31
Last Modified: 21 Apr 2016 17:06
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