Schizosaccharomyces selective differential media

Benito Saez, Santiago and Galvez Patón, Laura and Palomero Rodriguez, Felipe and Calderon Fernandez, Fernando and Morata Barrado, Antonio and Palmero Llamas, Daniel and Suarez Lepe, Jose Antonio (2013). Schizosaccharomyces selective differential media. "African Journal of Microbiology Research", v. 7 (n. 24); pp. 3026-3036. ISSN 1996-0808.


Title: Schizosaccharomyces selective differential media
  • Benito Saez, Santiago
  • Galvez Patón, Laura
  • Palomero Rodriguez, Felipe
  • Calderon Fernandez, Fernando
  • Morata Barrado, Antonio
  • Palmero Llamas, Daniel
  • Suarez Lepe, Jose Antonio
Item Type: Article
Título de Revista/Publicación: African Journal of Microbiology Research
Date: 2013
Volume: 7
Faculty: E.U.I.T. Agrícolas (UPM)
Department: Producción Vegetal: Botánica y Protección Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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This study discusses the optimisation of a selectiv e and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms) to select individuals from this genus for industrial purposes, especially in light of the recent approval of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main thephysiological characteristics of this species, such as its high resistance to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the resistance of the genus to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid compared to possible microorganisms which can give rise to false positive results. Malic acid was used as a differential fact or due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey.

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Item ID: 19114
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Deposited by: Memoria Investigacion
Deposited on: 28 Aug 2013 11:05
Last Modified: 21 Apr 2016 17:21
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