Developmental Expression of Kv Potassium Channels at the Axon Initial Segment of Cultured Hippocampal Neurons

Sánchez-Ponce, Diana and Felipe Oroquieta, Javier de and Garrido, Juan José and Muñoz Céspedes, Alberto (2012). Developmental Expression of Kv Potassium Channels at the Axon Initial Segment of Cultured Hippocampal Neurons. "Plos One", v. 7 (n. 10); pp.. ISSN 1932-6203. https://doi.org/10.1371/journal.pone.0048557.

Description

Title: Developmental Expression of Kv Potassium Channels at the Axon Initial Segment of Cultured Hippocampal Neurons
Author/s:
  • Sánchez-Ponce, Diana
  • Felipe Oroquieta, Javier de
  • Garrido, Juan José
  • Muñoz Céspedes, Alberto
Item Type: Article
Título de Revista/Publicación: Plos One
Date: 2012
ISSN: 1932-6203
Volume: 7
Subjects:
Faculty: Centro de Tecnología Biomédica (CTB) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB) | Preview

Abstract

Axonal outgrowth and the formation of the axon initial segment (AIS) are early events in the acquisition of neuronal polarity. The AIS is characterized by a high concentration of voltage-dependent sodium and potassium channels. However, the specific ion channel subunits present and their precise localization in this axonal subdomain vary both during development and among the types of neurons, probably determining their firing characteristics in response to stimulation. Here, we characterize the developmental expression of different subfamilies of voltage-gated potassium channels in the AISs of cultured mouse hippocampal neurons, including subunits Kv1.2, Kv2.2 and Kv7.2. In contrast to the early appearance of voltage-gated sodium channels and the Kv7.2 subunit at the AIS, Kv1.2 and Kv2.2 subunits were tethered at the AIS only after 10 days in vitro. Interestingly, we observed different patterns of Kv1.2 and Kv2.2 subunit expression, with each confined to distinct neuronal populations. The accumulation of Kv1.2 and Kv2.2 subunits at the AIS was dependent on ankyrin G tethering, it was not affected by disruption of the actin cytoskeleton and it was resistant to detergent extraction, as described previously for other AIS proteins. This distribution of potassium channels in the AIS further emphasizes the heterogeneity of this structure in different neuronal populations, as proposed previously, and suggests corresponding differences in action potential regulation.

More information

Item ID: 19828
DC Identifier: http://oa.upm.es/19828/
OAI Identifier: oai:oa.upm.es:19828
DOI: 10.1371/journal.pone.0048557
Official URL: http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048557
Deposited by: Memoria Investigacion
Deposited on: 11 Oct 2013 16:16
Last Modified: 21 Apr 2016 21:24
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM