Connexin-43 in the osteogenic BM niche regulates its cellular composition and the bidirectional traffic of hematopoietic stem cells and progenitors

Gonzalez Nieto, Daniel and Lina, Li and Köhler, Anja and Ghiaur, Gabriel and Hishikawa, Eri and Sengupta, Amitava and Madhu, Malav and Arnett, Jorden and Santho, Rebecca and Dunn, Susan K. and Fishman, Glenn I. and Gutstein, David E. and Civitelli, Roberto and Barrio, Luis C. and Gunzer, Matthias and Cancelas Pérez, José A. (2012). Connexin-43 in the osteogenic BM niche regulates its cellular composition and the bidirectional traffic of hematopoietic stem cells and progenitors. "Blood", v. 119 (n. 22); pp. 5144-5154. ISSN 0006-4971. https://doi.org/10.1182/blood-2011-07-368506.

Description

Title: Connexin-43 in the osteogenic BM niche regulates its cellular composition and the bidirectional traffic of hematopoietic stem cells and progenitors
Author/s:
  • Gonzalez Nieto, Daniel
  • Lina, Li
  • Köhler, Anja
  • Ghiaur, Gabriel
  • Hishikawa, Eri
  • Sengupta, Amitava
  • Madhu, Malav
  • Arnett, Jorden
  • Santho, Rebecca
  • Dunn, Susan K.
  • Fishman, Glenn I.
  • Gutstein, David E.
  • Civitelli, Roberto
  • Barrio, Luis C.
  • Gunzer, Matthias
  • Cancelas Pérez, José A.
Item Type: Article
Título de Revista/Publicación: Blood
Date: May 2012
ISSN: 0006-4971
Volume: 119
Subjects:
Freetext Keywords: Connexin-43, hematopoietic stem cells and progenitors, osteoblasts, osteogenic progenitors, stroma, bone marrow
Faculty: E.T.S.I. Telecomunicación (UPM)
Department: Tecnología Fotónica [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Connexin-43 (Cx43), a gap junction protein involved in control of cell proliferation, differentiation and migration, has been suggested to have a role in hematopoiesis. Cx43 is highly expressed in osteoblasts and osteogenic progenitors (OB/P). To elucidate the biologic function of Cx43 in the hematopoietic microenvironment (HM) and its influence in hematopoietic stem cell (HSC) activity, we studied the hematopoietic function in an in vivo model of constitutive deficiency of Cx43 in OB/P. The deficiency of Cx43 in OB/P cells does not impair the steady state hematopoiesis, but disrupts the directional trafficking of HSC/progenitors (Ps) between the bone marrow (BM) and peripheral blood (PB). OB/P Cx43 is a crucial positive regulator of transstromal migration and homing of both HSCs and progenitors in an irradiated microenvironment. However, OB/P Cx43 deficiency in nonmyeloablated animals does not result in a homing defect but induces increased endosteal lodging and decreased mobilization of HSC/Ps associated with proliferation and expansion of Cxcl12-secreting mesenchymal/osteolineage cells in the BM HM in vivo. Cx43 controls the cellular content of the BM osteogenic microenvironment and is required for homing of HSC/Ps in myeloablated animals

More information

Item ID: 19830
DC Identifier: http://oa.upm.es/19830/
OAI Identifier: oai:oa.upm.es:19830
DOI: 10.1182/blood-2011-07-368506
Official URL: http://bloodjournal.hematologylibrary.org/content/119/22/5144
Deposited by: Memoria Investigacion
Deposited on: 21 Sep 2013 11:29
Last Modified: 21 Apr 2016 21:25
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