Colocalization of α-actinin and Synaptopodin in the Pyramidal Cell Axon Initial Segment

Sánchez-Ponce, Diana and Blázquez-Llorca, Lidia and Felipe Oroquieta, Javier de and Garrido, Juan José and Muñoz Céspedes, Alberto (2012). Colocalization of α-actinin and Synaptopodin in the Pyramidal Cell Axon Initial Segment. "Cerebral Cortex", v. 22 (n. 7); pp. 1648-1661. ISSN 1047-3211. https://doi.org/10.1093/cercor/bhr251.

Description

Title: Colocalization of α-actinin and Synaptopodin in the Pyramidal Cell Axon Initial Segment
Author/s:
  • Sánchez-Ponce, Diana
  • Blázquez-Llorca, Lidia
  • Felipe Oroquieta, Javier de
  • Garrido, Juan José
  • Muñoz Céspedes, Alberto
Item Type: Article
Título de Revista/Publicación: Cerebral Cortex
Date: July 2012
ISSN: 1047-3211
Volume: 22
Subjects:
Faculty: Centro de Tecnología Biomédica (CTB) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The cisternal organelle that resides in the axon initial segment (AIS) of neocortical and hippocampal pyramidal cells is thought to be involved in regulating the Ca(2+) available to maintain AIS scaffolding proteins, thereby preserving normal AIS structure and function. Through immunocytochemistry and correlative light and electron microscopy, we show here that the actin-binding protein ?-actinin is present in the typical cistenal organelle of rodent pyramidal neurons as well as in a large structure in the AIS of a subpopulation of layer V pyramidal cells that we have called the "giant saccular organelle." Indeed, this localization of ?-actinin in the AIS is dependent on the integrity of the actin cytoskeleton. Moreover, in the cisternal organelle of cultured hippocampal neurons, ?-actinin colocalizes extensively with synaptopodin, a protein that interacts with both actin and ?-actinin, and they appear concomitantly during the development of these neurons. Together, these results indicate that ?-actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS.

More information

Item ID: 19831
DC Identifier: http://oa.upm.es/19831/
OAI Identifier: oai:oa.upm.es:19831
DOI: 10.1093/cercor/bhr251
Official URL: http://cercor.oxfordjournals.org/content/22/7/1648.full
Deposited by: Memoria Investigacion
Deposited on: 14 Oct 2013 15:08
Last Modified: 21 Apr 2016 21:25
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