Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation.

Ahkami, Amir H. and Melzer, Michael and Ghaffari, Mohammad R. and Pollmann, Stephan and Ghorbani, Majid and Shahinnia, Fahimeh and Hajirezaei, Mohammad R. and Druege, Uwe (2013). Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation.. "Planta", v. 238 (n. 3); pp. 499-517. ISSN 0032-0935. https://doi.org/10.1007/s00425-013-1907-z.

Description

Title: Distribution of indole-3-acetic acid in Petunia hybrida shoot tip cuttings and relationship between auxin transport, carbohydrate metabolism and adventitious root formation.
Author/s:
  • Ahkami, Amir H.
  • Melzer, Michael
  • Ghaffari, Mohammad R.
  • Pollmann, Stephan
  • Ghorbani, Majid
  • Shahinnia, Fahimeh
  • Hajirezaei, Mohammad R.
  • Druege, Uwe
Item Type: Article
Título de Revista/Publicación: Planta
Date: September 2013
ISSN: 0032-0935
Volume: 238
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

To determine the contribution of polar auxin transport (PAT) to auxin accumulation and to adventitious root (AR) formation in the stem base of Petunia hybrida shoot tip cuttings, the level of indole-3-acetic acid (IAA) was monitored in non-treated cuttings and cuttings treated with the auxin transport blocker naphthylphthalamic acid (NPA) and was complemented with precise anatomical studies. The temporal course of carbohydrates, amino acids and activities of controlling enzymes was also investigated. Analysis of initial spatial IAA distribution in the cuttings revealed that approximately 40 and 10% of the total IAA pool was present in the leaves and the stem base as rooting zone, respectively. A negative correlation existed between leaf size and IAA concentration. After excision of cuttings, IAA showed an early increase in the stem base with two peaks at 2 and 24h post excision and, thereafter, a decline to low levels. This was mirrored by the expression pattern of the auxin-responsive GH3 gene. NPA treatment completely suppressed the 24-h peak of IAA and severely inhibited root formation. It also reduced activities of cell wall and vacuolar invertases in the early phase of AR formation and inhibited the rise of activities of glucose-6-phosphate dehydrogenase and phosphofructokinase during later stages. We propose a model in which spontaneous AR formation in Petunia cuttings is dependent on PAT and on the resulting 24-h peak of IAA in the rooting zone, where it induces early cellular events and also stimulates sink establishment. Subsequent root development stimulates glycolysis and the pentosephosphate pathway

More information

Item ID: 19898
DC Identifier: http://oa.upm.es/19898/
OAI Identifier: oai:oa.upm.es:19898
DOI: 10.1007/s00425-013-1907-z
Official URL: http://link.springer.com/article/10.1007/s00425-013-1907-z
Deposited by: Memoria Investigacion
Deposited on: 12 Sep 2013 17:08
Last Modified: 30 Sep 2014 22:56
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