Synthetic seed production from encapsulated somatic embryos of cork oak (Quercus suber L.) and automated growth monitoring.

Pintos López, Beatriz and Bueno Pérez, M.Angeles and Cuenca Valera, Beatriz and Manzanera de la Vega, José Antonio (2008). Synthetic seed production from encapsulated somatic embryos of cork oak (Quercus suber L.) and automated growth monitoring.. "Plant Cell Tissue and Organ Culture", v. 95 (n. 2); pp. 217-225. ISSN 0167-6857. https://doi.org/10.1007/s11240-008-9435-4..

Description

Title: Synthetic seed production from encapsulated somatic embryos of cork oak (Quercus suber L.) and automated growth monitoring.
Author/s:
  • Pintos López, Beatriz
  • Bueno Pérez, M.Angeles
  • Cuenca Valera, Beatriz
  • Manzanera de la Vega, José Antonio
Item Type: Article
Título de Revista/Publicación: Plant Cell Tissue and Organ Culture
Date: November 2008
ISSN: 0167-6857
Volume: 95
Subjects:
Freetext Keywords: Automated monitoring, Cork oak, Encapsulation, Quercus suber L., Seed storage, Synthetic seeds.
Faculty: E.T.S.I. Montes (UPM)
Department: Silvopascicultura [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Cork oak (Quercus suber) somatic embryos were coatedwith alginate for the production of synthetic seeds and their storability for commercialization was investigated. Also, the automatic monitoring of somatic embryo growth with a digital system of image capture was tested.Apower regressionmodelwasfitted between size and fresh weight (Adjusted R-squared = 0.96). This method permitted growth assessment without contamination risk and opens the possibility of an automated control of culture growth for the future up scaling of plant production. Conversion rate of synthetic seedswas higher onmediumsupplementedwithmineral nutrients than onmediumwithout nutrients. Also, when the somatic embryos were coated without mineral nutrients added to the capsule, conversion rate was significantly lower. The addition of sucrose to the capsule had no significant effect on the conversion rate. No differences were recorded between 50 and 100 mM CaCl2 for capsule complexation. Synthetic seeds were cold stored at 4_C for two months without significant loss of conversion capacity. The present study reports the first attempts to determine optimal storage time and conditions for conversion of encapsulated somatic embryos of cork oak.

More information

Item ID: 2127
DC Identifier: http://oa.upm.es/2127/
OAI Identifier: oai:oa.upm.es:2127
DOI: 10.1007/s11240-008-9435-4.
Official URL: http://www.springerlink.com/content/100327/
Deposited by: Memoria Investigacion
Deposited on: 29 Apr 2010 11:05
Last Modified: 25 May 2015 14:08
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