A new ultrasonic signal amplification method for detection bacteria

Shukla, Shiva Kant and Resa López, Pablo and Sierra Sánchez, Carlos and Urréjola Soriano, José and Elvira Segura, Luis (2012). A new ultrasonic signal amplification method for detection bacteria. "Measurement Science and Technology IOP Science", v. 23 (n. 10); pp.. ISSN 1361-6501. https://doi.org/10.1088/0957-0233/23/10/105701.

Description

Title: A new ultrasonic signal amplification method for detection bacteria
Author/s:
  • Shukla, Shiva Kant
  • Resa López, Pablo
  • Sierra Sánchez, Carlos
  • Urréjola Soriano, José
  • Elvira Segura, Luis
Item Type: Article
Título de Revista/Publicación: Measurement Science and Technology IOP Science
Date: 14 August 2012
ISSN: 1361-6501
Volume: 23
Subjects:
Freetext Keywords: pulse-echo, rapid microorganism detection, catalase activity, bubble trapping medium
Faculty: Centro de Acústica Aplicada y Evaluación no Destructiva (CAEND) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (858kB) | Preview

Abstract

A new method is presented that increases the sensitivity of ultrasound-based techniques for detection of bacteria. The technique was developed for the detection of catalase-positive microorganisms. It uses a bubble trapping medium containing hydrogen peroxide that is mixed with the sample for microbiological evaluation. The enzyme catalase is present in catalase-positive bacteria, which induces a rapid hydrolysis of hydrogen peroxide, forming bubbles which remain in the medium. This reaction results in the amplification of the mechanical changes that the microorganisms produce in the medium. The effect can be detected by means of ultrasonic wave amplitude continuous measurement since the bubbles increase the ultrasonic attenuation significantly. It is shown that microorganism concentrations of the order of 105 cells ml−1 can be detected using this method. This allows an improvement of three orders of magnitude in the ultrasonic detection threshold of microorganisms in conventional culture media, and is competitive with modern rapid microbiological methods. It can also be used for the characterization of the enzymatic activity.

More information

Item ID: 21689
DC Identifier: http://oa.upm.es/21689/
OAI Identifier: oai:oa.upm.es:21689
DOI: 10.1088/0957-0233/23/10/105701
Official URL: http://iopscience.iop.org/0957-0233/23/10/105701/article
Deposited by: Memoria Investigacion
Deposited on: 28 Apr 2015 18:11
Last Modified: 28 Apr 2015 18:11
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM