A new ultrasonic signal amplification method for detection bacteria

Shukla, Shiva Kant; Resa López, Pablo; Sierra Sánchez, Carlos; Urréjola Soriano, José y Elvira Segura, Luis (2012). A new ultrasonic signal amplification method for detection bacteria. "Measurement Science and Technology IOP Science", v. 23 (n. 10); pp.. ISSN ISSN 1361-6501.

Descripción

Título: A new ultrasonic signal amplification method for detection bacteria
Autor/es:
  • Shukla, Shiva Kant
  • Resa López, Pablo
  • Sierra Sánchez, Carlos
  • Urréjola Soriano, José
  • Elvira Segura, Luis
Tipo de Documento: Artículo
Título de Revista/Publicación: Measurement Science and Technology IOP Science
Fecha: 14 Agosto 2012
Volumen: 23
Materias:
Palabras Clave Informales: pulse-echo, rapid microorganism detection, catalase activity, bubble trapping medium
Escuela: Centro de Acústica Aplicada y Evaluación no Destructiva (CAEND) (UPM)
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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URL Oficial: http://iopscience.iop.org/0957-0233/23/10/105701/article

Resumen

A new method is presented that increases the sensitivity of ultrasound-based techniques for detection of bacteria. The technique was developed for the detection of catalase-positive microorganisms. It uses a bubble trapping medium containing hydrogen peroxide that is mixed with the sample for microbiological evaluation. The enzyme catalase is present in catalase-positive bacteria, which induces a rapid hydrolysis of hydrogen peroxide, forming bubbles which remain in the medium. This reaction results in the amplification of the mechanical changes that the microorganisms produce in the medium. The effect can be detected by means of ultrasonic wave amplitude continuous measurement since the bubbles increase the ultrasonic attenuation significantly. It is shown that microorganism concentrations of the order of 105 cells ml−1 can be detected using this method. This allows an improvement of three orders of magnitude in the ultrasonic detection threshold of microorganisms in conventional culture media, and is competitive with modern rapid microbiological methods. It can also be used for the characterization of the enzymatic activity.

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ID de Registro: 21689
Identificador DC: http://oa.upm.es/21689/
Identificador OAI: oai:oa.upm.es:21689
Depositado por: Memoria Investigacion
Depositado el: 28 Abr 2015 18:11
Ultima Modificación: 28 Abr 2015 18:11
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