Transcriptional changes and oxidative stress associated with the synergistic interaction between potato virus X and potato virus Y and their relationship with symptom expression.

García Marcos, Alberto and Pacheco Piña, Remedios and Martiánez, Justo and González Jara, Pablo and Díaz Ruíz, José Ramón and Tenllado, Francisco (2009). Transcriptional changes and oxidative stress associated with the synergistic interaction between potato virus X and potato virus Y and their relationship with symptom expression.. "Molecular Plant-Microbe Interactions", v. 22 (n. 11); pp. 1431-1444. ISSN 0894-0282. https://doi.org/10.1094/MPMI-22-11-1431.

Description

Title: Transcriptional changes and oxidative stress associated with the synergistic interaction between potato virus X and potato virus Y and their relationship with symptom expression.
Author/s:
  • García Marcos, Alberto
  • Pacheco Piña, Remedios
  • Martiánez, Justo
  • González Jara, Pablo
  • Díaz Ruíz, José Ramón
  • Tenllado, Francisco
Item Type: Article
Título de Revista/Publicación: Molecular Plant-Microbe Interactions
Date: November 2009
ISSN: 0894-0282
Volume: 22
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (3MB) | Preview

Abstract

Many virus diseases of economic importance to agriculture result from mixtures of different pathogens invading the host at a given time. This contrasts with the relatively scarce studies available on the molecular events associated with virus---host interactions in mixed infections. Compared with single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) resulted in increased systemic symptoms (synergism) that led to necrosis of the newly emerging leaves and death of the plant. A comparative transcriptional analysis was undertaken to identify quantitative and qualitative differences in gene expression during this synergistic infection and correlate these changes with the severe symptoms it caused. Global transcription profiles of doubly infected leaves were compared with those from singly infected leaves using gene ontology enrichment analysis and metabolic pathway annotator software. Functional gene categories altered by the double infection comprise suites of genes regulated coordinately, which are associated with chloroplast functions (downregulated), protein synthesis and degradation (upregulated), carbohydrate metabolism (upregulated), and response to biotic stimulus and stress (upregulated). The expressions of reactive oxygen species?generating enzymes as well as several mitogen-activated protein kinases were also significantly induced. Accordingly, synergistic infection induced a severe oxidative stress in N. benthamiana leaves, as judged by increases in lipid peroxidation and by the generation of superoxide radicals in chloroplasts, which correlated with the misregulation of antioxidative genes in microarray data. Interestingly, expression of genes encoding oxylipin biosynthesis was uniquely upregulated by the synergistic infection. Virus-induced gene silencing of ?-dioxygenase1 delayed cell death during PVX?PVY infection.

More information

Item ID: 22626
DC Identifier: http://oa.upm.es/22626/
OAI Identifier: oai:oa.upm.es:22626
DOI: 10.1094/MPMI-22-11-1431
Official URL: http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-22-11-1431
Deposited by: Memoria Investigacion
Deposited on: 25 Jul 2014 13:16
Last Modified: 21 Apr 2016 17:36
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM