A quantitative analysis of complementation of deleterious mutants in plant virus populations

Fraile Pérez, Aurora and Sacristán Benayas, Soledad and Garcia-Arenal Rodriguez, Fernando (2008). A quantitative analysis of complementation of deleterious mutants in plant virus populations. "Spanish Journal of Agricultural Research", v. 6 (n. Specia); pp. 195-200. ISSN 1695-971-X.


Title: A quantitative analysis of complementation of deleterious mutants in plant virus populations
  • Fraile Pérez, Aurora
  • Sacristán Benayas, Soledad
  • Garcia-Arenal Rodriguez, Fernando
Item Type: Article
Título de Revista/Publicación: Spanish Journal of Agricultural Research
Date: February 2008
Volume: 6
Freetext Keywords: mutant complementation, selection, Tobacco mosaic virus, virus evolution. - complementación de mutantes, evolución de virus, selección, virus del mosaico del tabaco.
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Complementation can be defined as the process by which the function affected by a mutation is provided in trans by fully competent genotypes. Complementation can, thus, counter the effects of selection of deleterious mutants. Complementation of mutants defective for replication, movement and transmission has been often described in experiments with viruses and, occasionally, has been reported to occur in their natural populations. However, the role of complementation in virus evolution has been overlooked. Here is provided a quantitative estimate of the efficiency of complementation, defined as the probability that a non-functional mutant accomplishes a function relative to a functional one. For this, the frequency of mutants of Tobacco mosaic virus (TMV) defective for cell to cell movement was estimated in wild type tobacco plants and in transgenic plants expressing the TMV movement protein (MP) from a transgene. Mutants lethal for cell-to-cell movement were complemented by wild-type TMV in the first case, and by the transgene-expressed MP in the second case. Assuming that complementation is fully efficient in the transgenic plants, a value for the efficiency of complementation of 0.34 was obtained. Thus, complementation can efficiently counter selection on lethal mutants, and may have an important role on virus evolution. Complementation may be relevant for management of viral diseases if the complemented deleterious mutation is linked to other functions affecting the pathogenicity or epidemiology of the virus. - La complementación puede definirse como el proceso por el cual una función afectada por una mutación es provista en trans por genotipos competentes para dicha función. La complementación de mutantes defectivos para replicación, movimiento o transmisión se ha descrito con frecuencia en poblaciones experimentales de virus y, ocasionalmente, en poblaciones de campo. Sin embargo, el papel de la complementación en la evolución de los virus no se ha considerado. En este trabajo presentamos una estima de la eficacia de la complementación, definida como la probabilidad de que un mutante no funcional realice la función respecto a un genotipo funcional. Para ello se estimó la frecuencia de mutantes del virus del mosaico del tabaco (TMV) defectivos para el movimiento de célula a célula en plantas silvestres de tabaco y en plantas transgénicas que expresan constitutivamente la proteína del movimiento (MP) de TMV. En el primer caso, los mutantes letales para movimiento serán complementados por genotipos silvestres de TMV, y en el segundo, por la MP transgénica. Suponiendo que la complementación en las plantas transgénicas es totalmente eficaz, se obtuvo un valor de la eficacia de complementación de 0,34. Esto muestra que la complementación puede contrarrestar eficazmente el efecto de la selección sobre mutantes deletéreos y tener un papel importante en la evolución de virus, con importantes implicaciones prácticas si la mutación deletérea complementada está ligada a otras funciones que afecten a la patogenicidad o epidemiología del virus.

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Item ID: 2600
DC Identifier: http://oa.upm.es/2600/
OAI Identifier: oai:oa.upm.es:2600
Deposited by: Memoria Investigacion
Deposited on: 18 Mar 2010 10:42
Last Modified: 20 Apr 2016 12:15
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