Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines.

Gomez Casado, Cristina and Garrido Arandia, M. and Gamboa, P.M. and Blanca-López, N. and Canto, G. and Varela, J. and Cuesta-Herranz, Javier and Pacios, Luis F. and Díaz Perales, Araceli and Tordesillas Villuendas, Leticia (2013). Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines.. "Clinical & Developmental Immunology", v. 2013 ; pp.. ISSN 1740-2522. https://doi.org/10.1155/2013/385615.

Description

Title: Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines.
Author/s:
  • Gomez Casado, Cristina
  • Garrido Arandia, M.
  • Gamboa, P.M.
  • Blanca-López, N.
  • Canto, G.
  • Varela, J.
  • Cuesta-Herranz, Javier
  • Pacios, Luis F.
  • Díaz Perales, Araceli
  • Tordesillas Villuendas, Leticia
Item Type: Article
Título de Revista/Publicación: Clinical & Developmental Immunology
Date: 2013
ISSN: 1740-2522
Volume: 2013
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Nowadays, treatment of food allergy only considered the avoidance of the specific food. However, the possibility of cross-reactivity makes this practice not very effective. Immunotherapy may exhibit as a good alternative to food allergy treatment. The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy. In this study, three mutants of Pru p 3, the principal allergen of peach, were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named as Pru p 3.01, Pru p 3.02, and Pru p 3.03. Pru p 3.01 showed very similar allergenic activity as the wild type by in vitro assays. However, Pru p 3.02 and Pru p 3.03 presented reduced IgE binding with respect to the native form, by in vitro, ex vivo, and in vivo assays. In addition, Pru p 3.03 had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3. Nevertheless, both Pru p 3.02 and Pru p 3.03 maintained the binding to IgG1 and their ability to activate T lymphocytes. Thus, Pru p 3.02 and Pru p 3.03 could be good candidates for potential immunotherapy in peach-allergic patients.

More information

Item ID: 26278
DC Identifier: http://oa.upm.es/26278/
OAI Identifier: oai:oa.upm.es:26278
DOI: 10.1155/2013/385615
Official URL: http://www.hindawi.com/journals/jir/2013/385615/
Deposited by: Memoria Investigacion
Deposited on: 23 Jun 2014 17:17
Last Modified: 22 Sep 2014 11:40
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