Effects of forage type on diversity in bacterial pellets isolated from liquid and solid phases of the rumen content in sheep and goats.

Abecilla, L. and Molina Alcaide, Eduarda and Cantalapiedra Hijar, G. and Soto, M. D. and Carro Travieso, María Dolores (2013). Effects of forage type on diversity in bacterial pellets isolated from liquid and solid phases of the rumen content in sheep and goats.. "Options Mediterranéennes", v. 107 ; pp. 9-15. ISSN 1016-121-X.

Description

Title: Effects of forage type on diversity in bacterial pellets isolated from liquid and solid phases of the rumen content in sheep and goats.
Author/s:
  • Abecilla, L.
  • Molina Alcaide, Eduarda
  • Cantalapiedra Hijar, G.
  • Soto, M. D.
  • Carro Travieso, María Dolores
Item Type: Article
Título de Revista/Publicación: Options Mediterranéennes
Date: 2013
Volume: 107
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Animal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Two sheep and two goats, fitted with a ruminal cannula, received two diets composed of 30% concentrate and 70% of either alfalfa hay (AL) or grass hay (GR) as forage in a two-period crossover design. Solid and liquid phases of the rumen were sampled from each animal immediately before feeding and 4 h post-feeding. Pellets containing solid associated bacteria (SAB) and liquid associated bacteria (LAB) were isolated from the corresponding ruminal phase and composited by time to obtain 2 pellets per animal (one SAB and one LAB) before DNA extraction. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S ribosomal DNA was used to analyze bacterial diversity. A total of 78 and 77 bands were detected in the DGGE gel from sheep and goats samples, respectively. There were 18 bands only found in the pellets from sheep fed AL-fed sheep and 7 found exclusively in samples from sheep fed the GR diet. In goats, 21 bands were found only in animals fed the AL diet and 17 were found exclusively in GR-fed ones. In all animals, feeding AL diet tended (P < 0.10) to promote greater NB and SI in LAB and SAB pellets compared with the GR diet. The dendrogram generated by the cluster analysis showed that in both animal species all samples can be included in two major clusters. The four SAB pellets within each animal species clustered together and the four LAB pellets grouped in a different cluster. Moreover, SAB and LAB clusters contained two clear subclusters according to forage type. Results show that in all animals bacterial diversity was more markedly affected by the ruminal phase (solid vs. liquid) than by the type of forage in the diet.

More information

Item ID: 26553
DC Identifier: http://oa.upm.es/26553/
OAI Identifier: oai:oa.upm.es:26553
Official URL: http://om.ciheam.org/om/pdf/a107/a107.pdf
Deposited by: Memoria Investigacion
Deposited on: 26 Jun 2014 13:23
Last Modified: 22 Sep 2014 11:41
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