Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.

Brito Lopez, Maria Belen and Toffanin, Annita and Prieto Carbajo, Rosa Isabel and Imperial Ródenas, Juan and Ruiz Argüeso, Tomas-Andres and Palacios Alberti, Jose Manuel (2008). Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.. "Molecular Plant-Microbe Interactions", v. 21 (n. 5); pp. 597-604. ISSN 0894-0282. https://doi.org/10.1094/MPMI -21-5-0597.

Description

Title: Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.
Author/s:
  • Brito Lopez, Maria Belen
  • Toffanin, Annita
  • Prieto Carbajo, Rosa Isabel
  • Imperial Ródenas, Juan
  • Ruiz Argüeso, Tomas-Andres
  • Palacios Alberti, Jose Manuel
Item Type: Article
Título de Revista/Publicación: Molecular Plant-Microbe Interactions
Date: May 2008
ISSN: 0894-0282
Volume: 21
Subjects:
Freetext Keywords: hup and hyp genes, nitrogen fixation.
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

TThe legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R. leguminosarum bacteroids from different hosts, with the exception of lentil (Lens culinaris). Transcription analysis showed that the NifA-regulated R. leguminosarum hydrogenase structural gene promoter (P1) is poorly induced in lentil root nodules. Replacement of the P1 promoter by the FnrN-dependent promoter of the fixN gene restored transcription of hup genes in lentil bacteroids, but not hydrogenase activity. In the PfixN-hupSL strain, additional copies of the hup gene cluster and nickel supplementation to lentil plants increased bacteroid hydrogenase activity. However, the level of activity in lentil still was significantly lower than in pea bacteroids, indicating that an additional factor is impairing hydrogenase expression inside lentil nodules. Immunological analysis revealed that lentil bacteroids contain reduced levels of both hydrogenase structural subunit HupL and nickel-binding protein HypB. Altogether, results indicate that hydrogenase expression is affected by the legume host at the level of both transcription of hydrogenase structural genes and biosynthesis or stability of nickelrelated proteins HypB and HupL, and suggest the existence of a plant-dependent mechanism that affects hydrogenase activity during the symbiosis by limiting nickel availability to the bacteroid.

More information

Item ID: 2689
DC Identifier: http://oa.upm.es/2689/
OAI Identifier: oai:oa.upm.es:2689
DOI: 10.1094/MPMI -21-5-0597
Official URL: http://apsjournals.apsnet.org/toc/mpmi/21/5
Deposited by: Memoria Investigacion
Deposited on: 26 Mar 2010 12:54
Last Modified: 20 Apr 2016 12:22
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