Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.

Brito Lopez, Maria Belen; Toffanin, Annita; Prieto Carbajo, Rosa Isabel; Imperial Ródenas, Juan; Ruiz Argüeso, Tomas-Andres y Palacios Alberti, Jose Manuel (2008). Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.. "Molecular Plant-Microbe Interactions", v. 21 (n. 5); pp. 597-604. ISSN 0894-0282. https://doi.org/10.1094/MPMI -21-5-0597.

Descripción

Título: Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.
Autor/es:
  • Brito Lopez, Maria Belen
  • Toffanin, Annita
  • Prieto Carbajo, Rosa Isabel
  • Imperial Ródenas, Juan
  • Ruiz Argüeso, Tomas-Andres
  • Palacios Alberti, Jose Manuel
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Plant-Microbe Interactions
Fecha: Mayo 2008
Volumen: 21
Materias:
Palabras Clave Informales: hup and hyp genes, nitrogen fixation.
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (275kB) | Vista Previa

Resumen

TThe legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R. leguminosarum bacteroids from different hosts, with the exception of lentil (Lens culinaris). Transcription analysis showed that the NifA-regulated R. leguminosarum hydrogenase structural gene promoter (P1) is poorly induced in lentil root nodules. Replacement of the P1 promoter by the FnrN-dependent promoter of the fixN gene restored transcription of hup genes in lentil bacteroids, but not hydrogenase activity. In the PfixN-hupSL strain, additional copies of the hup gene cluster and nickel supplementation to lentil plants increased bacteroid hydrogenase activity. However, the level of activity in lentil still was significantly lower than in pea bacteroids, indicating that an additional factor is impairing hydrogenase expression inside lentil nodules. Immunological analysis revealed that lentil bacteroids contain reduced levels of both hydrogenase structural subunit HupL and nickel-binding protein HypB. Altogether, results indicate that hydrogenase expression is affected by the legume host at the level of both transcription of hydrogenase structural genes and biosynthesis or stability of nickelrelated proteins HypB and HupL, and suggest the existence of a plant-dependent mechanism that affects hydrogenase activity during the symbiosis by limiting nickel availability to the bacteroid.

Más información

ID de Registro: 2689
Identificador DC: http://oa.upm.es/2689/
Identificador OAI: oai:oa.upm.es:2689
Identificador DOI: 10.1094/MPMI -21-5-0597
URL Oficial: http://apsjournals.apsnet.org/toc/mpmi/21/5
Depositado por: Memoria Investigacion
Depositado el: 26 Mar 2010 12:54
Ultima Modificación: 20 Abr 2016 12:22
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM