Novel arrangement of enhancer sequences for NifA-dependent activation of the hydrogenase gene promoter in Rhizobium leguminosarum bv. viciae

Martínez González, Marta and Colombo Rodriguez, María Victoria and Palacios Alberti, Jose Manuel and Imperial Ródenas, Juan and Ruiz Argüeso, Tomas-Andres (2008). Novel arrangement of enhancer sequences for NifA-dependent activation of the hydrogenase gene promoter in Rhizobium leguminosarum bv. viciae. "Journal of Bacteriology", v. 190 (n. 9); pp. 3185-3191. ISSN 0021-9193. https://doi.org/10.1128/JB.00107-08.

Description

Title: Novel arrangement of enhancer sequences for NifA-dependent activation of the hydrogenase gene promoter in Rhizobium leguminosarum bv. viciae
Author/s:
  • Martínez González, Marta
  • Colombo Rodriguez, María Victoria
  • Palacios Alberti, Jose Manuel
  • Imperial Ródenas, Juan
  • Ruiz Argüeso, Tomas-Andres
Item Type: Article
Título de Revista/Publicación: Journal of Bacteriology
Date: May 2008
ISSN: 0021-9193
Volume: 190
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The transcriptional activation of the NifA-dependent !54 promoter of the Rhizobium leguminosarum hydrogenase structural genes hupSL (P1) has been studied through gel retardation analysis and detailed mutagenesis. Gel retardation analysis indicated the existence of a physical interaction between NifA and the promoter. Extensive mutagenesis followed by in vivo expression analysis showed that three sequences of 4 bases each ("170 ACAA "167, "161 ACAA "158, and "145 TTGT "142) are required for maximal stimulation of in vivo transcription of the P1 promoter. The arrangement of these upstream activating sequences (ACAA N5 ACAA N12 TTGT) differs from the canonical 5!ACA N10 TGT 3! UAS structure involved in NifA-dependent activation of nif/fix genes. Mutant promoter analysis indicated that the relative contribution of each of these sequences to P1 promoter activity increases with its proximity to the transcription start site. Analysis of double mutants altered in two out of the three enhancer sequences suggests that each of these sequences functions in NifA-dependent activation of the P1 promoter in an independent but cooperative mode. The similarities and differences between cis elements of hup and nif/fix promoters suggest that the structure of the P1 promoter has adapted to activation by NifA in order to coexpress hydrogenase and nitrogenase activities in legume nodules.

More information

Item ID: 2708
DC Identifier: http://oa.upm.es/2708/
OAI Identifier: oai:oa.upm.es:2708
DOI: 10.1128/JB.00107-08
Official URL: http://jb.asm.org/content/vol190/issue9/index.dtl
Deposited by: Memoria Investigacion
Deposited on: 06 Apr 2010 10:02
Last Modified: 20 Apr 2016 12:23
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