Brenneria quercina and Serratia spp. isolated from Spanish oak trees: molecular characterization and PCR development

Poza Carrion, Cesar and Aguilar, I. and Gallego Rodriguez, Francisco Javier and Nuñez Moreno, Y. and González Biosca, Elena and González, R. and Lopez, M. M. and Rodriguez Palenzuela, Pablo (2008). Brenneria quercina and Serratia spp. isolated from Spanish oak trees: molecular characterization and PCR development. "Plant Pathology", v. 57 (n. 2); pp. 308-319. ISSN 0032-0862.

Description

Title: Brenneria quercina and Serratia spp. isolated from Spanish oak trees: molecular characterization and PCR development
Author/s:
  • Poza Carrion, Cesar
  • Aguilar, I.
  • Gallego Rodriguez, Francisco Javier
  • Nuñez Moreno, Y.
  • González Biosca, Elena
  • González, R.
  • Lopez, M. M.
  • Rodriguez Palenzuela, Pablo
Item Type: Article
Título de Revista/Publicación: Plant Pathology
Date: April 2008
Volume: 57
Subjects:
Freetext Keywords: drippy nut disease, Quercus ilex, Quercus pyrenaica, rep-PCR detection and diagnosis.
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Brenneria quercina has been reported as one of the causal agents of oak decline in Spain. To investigate the bacterial variability of this pathogen from different Spanish oak forests, a collection of 38 bacterial isolates from seven geographic locations and from different oak species was analysed by sequencing 16S rDNA and rep-PCR fingerprinting. All Spanish isolates of B. quercina were grouped by rep-PCR into a homogenous cluster that differed significantly from B. quercina reference strains from California. 16S rDNA analysis revealed that 34 out of 38 isolates were Brenneria . However, four isolates belonged to the genus Serratia , suggesting that this bacterium could cause cankers in oak trees. The information obtained by rep-PCR fingerprint analysis was used to develop PCR primers for the sensitive and specific detection of B. quercina from infected plant tissues. Pathogenicity tests performed with Brenneria and Serratia isolates showed that both were able to grow and cause cankers in oak trees.

More information

Item ID: 2800
DC Identifier: http://oa.upm.es/2800/
OAI Identifier: oai:oa.upm.es:2800
Official URL: http://www3.interscience.wiley.com/journal/119395492/issue
Deposited by: Memoria Investigacion
Deposited on: 20 Apr 2010 11:11
Last Modified: 20 Apr 2016 12:27
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