Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi

Llorente, Francisco; Muskett, Paul; Sánchez Vallet, Andrea; López, Gemma; Ramos, Brisa; Sánchez Rodríguez, Clara; Jordá Miró, Lucía; Parker, Jane y Molina Fernández, Antonio (2008). Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi. "Molecular Plant", v. 1 (n. 3); pp. 496-509. ISSN 1674-2052. https://doi.org/10.1093/mp/ssn025.

Descripción

Título: Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi
Autor/es:
  • Llorente, Francisco
  • Muskett, Paul
  • Sánchez Vallet, Andrea
  • López, Gemma
  • Ramos, Brisa
  • Sánchez Rodríguez, Clara
  • Jordá Miró, Lucía
  • Parker, Jane
  • Molina Fernández, Antonio
Tipo de Documento: Artículo
Título de Revista/Publicación: Molecular Plant
Fecha: Mayo 2008
Volumen: 1
Materias:
Palabras Clave Informales: plant defense; innate immunity; necrotrophic fungi; auxin signaling; proteasome.
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

In plants, resistance to necrotrophic pathogens depends on the interplay between different hormone systems, such as those regulated by salicylic acid (SA), jasmonic acid (JA), ethylene, and abscisic acid. Repression of auxin signaling by the SA pathway was recently shown to contribute to antibacterial resistance. Here, we demonstrate that Arabidopsis auxin signaling mutants axr1, axr2, and axr6 that have defects in the auxin-stimulated SCF (Skp1¿Cullin¿ F-box) ubiquitination pathway exhibit increased susceptibility to the necrotrophic fungi Plectosphaerella cucumerina and Botrytis cinerea. Also, stabilization of the auxin transcriptional repressor AXR3 that is normally targeted for removal by the SCF-ubiquitin/proteasome machinery occurs upon P. cucumerina infection. Pharmacological inhibition of auxin transport or proteasome function each compromise necrotroph resistance of wild-type plants to a similar extent as in non-treated auxin response mutants. These results suggest that auxin signaling is important for resistance to the necrotrophic fungi P. cucumerina and B. cinerea. SGT1b (one of two Arabidopsis SGT1 genes encoding HSP90/HSC70 co-chaperones) promotes the functions of SCF E3-ubiquitin ligase complexes in auxin and JA responses and resistance conditioned by certain Resistance (R) genes to biotrophic pathogens. We find that sgt1b mutants are as resistant to P. cucumerina as wild-type plants. Conversely, auxin/SCF signaling mutants are uncompromised in RPP4-triggered resistance to the obligate biotrophic oomycete, Hyaloperonospora parasitica. Thus, the predominant action of SGT1b in R gene-conditioned resistance to oomycetes appears to be at a site other than assisting SCF E3-ubiquitin ligases. However, genetic additivity of sgt1b axr1 double mutants in susceptibility to H. parasitica suggests that SCF-mediated ubiquitination contributes to limiting biotrophic pathogen colonization once plant¿pathogen compatibility is established.

Más información

ID de Registro: 2852
Identificador DC: http://oa.upm.es/2852/
Identificador OAI: oai:oa.upm.es:2852
Identificador DOI: 10.1093/mp/ssn025
URL Oficial: http://mplant.oxfordjournals.org/content/vol1/issue3/
Depositado por: Memoria Investigacion
Depositado el: 14 Abr 2010 08:38
Ultima Modificación: 20 Abr 2016 12:29
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