Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi

Llorente, Francisco and Muskett, Paul and Sánchez Vallet, Andrea and López, Gemma and Ramos, Brisa and Sánchez Rodríguez, Clara and Jordá Miró, Lucía and Parker, Jane and Molina Fernández, Antonio (2008). Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi. "Molecular Plant", v. 1 (n. 3); pp. 496-509. ISSN 1674-2052. https://doi.org/10.1093/mp/ssn025.

Description

Title: Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi
Author/s:
  • Llorente, Francisco
  • Muskett, Paul
  • Sánchez Vallet, Andrea
  • López, Gemma
  • Ramos, Brisa
  • Sánchez Rodríguez, Clara
  • Jordá Miró, Lucía
  • Parker, Jane
  • Molina Fernández, Antonio
Item Type: Article
Título de Revista/Publicación: Molecular Plant
Date: May 2008
ISSN: 1674-2052
Volume: 1
Subjects:
Freetext Keywords: plant defense; innate immunity; necrotrophic fungi; auxin signaling; proteasome.
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In plants, resistance to necrotrophic pathogens depends on the interplay between different hormone systems, such as those regulated by salicylic acid (SA), jasmonic acid (JA), ethylene, and abscisic acid. Repression of auxin signaling by the SA pathway was recently shown to contribute to antibacterial resistance. Here, we demonstrate that Arabidopsis auxin signaling mutants axr1, axr2, and axr6 that have defects in the auxin-stimulated SCF (Skp1¿Cullin¿ F-box) ubiquitination pathway exhibit increased susceptibility to the necrotrophic fungi Plectosphaerella cucumerina and Botrytis cinerea. Also, stabilization of the auxin transcriptional repressor AXR3 that is normally targeted for removal by the SCF-ubiquitin/proteasome machinery occurs upon P. cucumerina infection. Pharmacological inhibition of auxin transport or proteasome function each compromise necrotroph resistance of wild-type plants to a similar extent as in non-treated auxin response mutants. These results suggest that auxin signaling is important for resistance to the necrotrophic fungi P. cucumerina and B. cinerea. SGT1b (one of two Arabidopsis SGT1 genes encoding HSP90/HSC70 co-chaperones) promotes the functions of SCF E3-ubiquitin ligase complexes in auxin and JA responses and resistance conditioned by certain Resistance (R) genes to biotrophic pathogens. We find that sgt1b mutants are as resistant to P. cucumerina as wild-type plants. Conversely, auxin/SCF signaling mutants are uncompromised in RPP4-triggered resistance to the obligate biotrophic oomycete, Hyaloperonospora parasitica. Thus, the predominant action of SGT1b in R gene-conditioned resistance to oomycetes appears to be at a site other than assisting SCF E3-ubiquitin ligases. However, genetic additivity of sgt1b axr1 double mutants in susceptibility to H. parasitica suggests that SCF-mediated ubiquitination contributes to limiting biotrophic pathogen colonization once plant¿pathogen compatibility is established.

More information

Item ID: 2852
DC Identifier: http://oa.upm.es/2852/
OAI Identifier: oai:oa.upm.es:2852
DOI: 10.1093/mp/ssn025
Official URL: http://mplant.oxfordjournals.org/content/vol1/issue3/
Deposited by: Memoria Investigacion
Deposited on: 14 Apr 2010 08:38
Last Modified: 20 Apr 2016 12:29
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