The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.

Putker, Florian and Tommasen-van Boxtel, Ria and Stork, Michiel and Rodríguez Herva, Jose Juan and Koster, Margot and Tommasen, Jan (2013). The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.. "Environmental Microbiology", v. 15 (n. 10); pp. 2658-2671. ISSN 1462-2912. https://doi.org/10.1111/1462-2920.12115.

Description

Title: The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.
Author/s:
  • Putker, Florian
  • Tommasen-van Boxtel, Ria
  • Stork, Michiel
  • Rodríguez Herva, Jose Juan
  • Koster, Margot
  • Tommasen, Jan
Item Type: Article
Título de Revista/Publicación: Environmental Microbiology
Date: October 2013
ISSN: 1462-2912
Volume: 15
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The genome of the Gram-negative bacterium Pseudomonas putida harbours a complete set of xcp genes for a type II protein secretion system (T2SS). This study shows that expression of these genes is induced under inorganic phosphate (Pi ) limitation and that the system enables the utilization of various organic phosphate sources. A phosphatase of the PhoX family, previously designated UxpB, was identified, which was produced under low Pi conditions and transported across the cell envelope in an Xcp-dependent manner demonstrating that the xcp genes encode an active T2SS. The signal sequence of UxpB contains a twin-arginine translocation (Tat) motif as well as a lipobox, and both processing by leader peptidase II and Tat dependency were experimentally confirmed. Two different tat gene clusters were detected in the P.?putida genome, of which one, named tat-1, is located adjacent to the uxpB and xcp genes. Both Tat systems appeared to be capable of transporting the UxpB protein. However, expression of the tat-1 genes was strongly induced by low Pi levels, indicating a function of this system in survival during Pi starvation.

More information

Item ID: 29008
DC Identifier: http://oa.upm.es/29008/
OAI Identifier: oai:oa.upm.es:29008
DOI: 10.1111/1462-2920.12115
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12115/abstract
Deposited by: Memoria Investigacion
Deposited on: 29 Jul 2014 14:09
Last Modified: 31 Oct 2014 23:56
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