The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.

Putker, Florian; Tommasen-van Boxtel, Ria; Stork, Michiel; Rodríguez Herva, Jose Juan; Koster, Margot y Tommasen, Jan (2013). The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.. "Environmental Microbiology", v. 15 (n. 10); pp. 2658-2671. ISSN 1462-2912. https://doi.org/10.1111/1462-2920.12115.

Descripción

Título: The type II secretion system (Xcp) of Pseudomonas putida is active and involved in the secretion of phosphatases.
Autor/es:
  • Putker, Florian
  • Tommasen-van Boxtel, Ria
  • Stork, Michiel
  • Rodríguez Herva, Jose Juan
  • Koster, Margot
  • Tommasen, Jan
Tipo de Documento: Artículo
Título de Revista/Publicación: Environmental Microbiology
Fecha: Octubre 2013
Volumen: 15
Materias:
Escuela: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

The genome of the Gram-negative bacterium Pseudomonas putida harbours a complete set of xcp genes for a type II protein secretion system (T2SS). This study shows that expression of these genes is induced under inorganic phosphate (Pi ) limitation and that the system enables the utilization of various organic phosphate sources. A phosphatase of the PhoX family, previously designated UxpB, was identified, which was produced under low Pi conditions and transported across the cell envelope in an Xcp-dependent manner demonstrating that the xcp genes encode an active T2SS. The signal sequence of UxpB contains a twin-arginine translocation (Tat) motif as well as a lipobox, and both processing by leader peptidase II and Tat dependency were experimentally confirmed. Two different tat gene clusters were detected in the P.?putida genome, of which one, named tat-1, is located adjacent to the uxpB and xcp genes. Both Tat systems appeared to be capable of transporting the UxpB protein. However, expression of the tat-1 genes was strongly induced by low Pi levels, indicating a function of this system in survival during Pi starvation.

Más información

ID de Registro: 29008
Identificador DC: http://oa.upm.es/29008/
Identificador OAI: oai:oa.upm.es:29008
Identificador DOI: 10.1111/1462-2920.12115
URL Oficial: http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12115/abstract
Depositado por: Memoria Investigacion
Depositado el: 29 Jul 2014 14:09
Ultima Modificación: 31 Oct 2014 23:56
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