Contraction speed of the actomyosin cytoskeleton in the absence of the cell membrane

Plaza Baonza, Gustavo Ramón and Uyeda, Taro Q. P. (2013). Contraction speed of the actomyosin cytoskeleton in the absence of the cell membrane. "Soft Matter", v. 9 (n. 17); pp. 4390-4400. ISSN 1744-683X. https://doi.org/10.1039/c3sm27867k.

Description

Title: Contraction speed of the actomyosin cytoskeleton in the absence of the cell membrane
Author/s:
  • Plaza Baonza, Gustavo Ramón
  • Uyeda, Taro Q. P.
Item Type: Article
Título de Revista/Publicación: Soft Matter
Date: 5 May 2013
ISSN: 1744-683X
Volume: 9
Subjects:
Faculty: E.T.S.I. Caminos, Canales y Puertos (UPM)
Department: Ciencia de los Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The contraction of the actomyosin cytoskeleton, which is produced by the sliding of myosin II along actin filaments, drives important cellular activities such as cytokinesis and cell migration. To explain the contraction velocities observed in such physiological processes, we have studied the contraction of intact cytoskeletons of Dictyostelium discoideum cells after removing the plasma membrane using Triton X-100. The technique developed in this work allows for the quantitative measurement of contraction rates of individual cytoskeletons. The relationship of the contraction rates with forces was analyzed using three different myosins with different in vitro sliding velocities. The cytoskeletons containing these myosins were always contractile and the contraction rate was correlated with the sliding velocity of the myosins. However, the values of the contraction rate were two to three orders of magnitude slower than expected from the in vitro sliding velocities of the myosins, presumably due to internal and external resistive forces. The contraction process also depended on actin cross-linking proteins. The lack of α-actinin increased the contraction rate 2-fold and reduced the capacity of the cytoskeleton to retain internal materials, while the lack of filamin resulted in the ATP-dependent disruption of the cytoskeleton. Interestingly, the myosin-dependent contraction rate of intact contractile rings is also reportedly much slower than the in vitro sliding velocity of myosin, and is similar to the contraction rates of cytoskeletons (different by only 2–3 fold), suggesting that the contraction of intact cells and cytoskeletons is limited by common mechanisms.

More information

Item ID: 29085
DC Identifier: http://oa.upm.es/29085/
OAI Identifier: oai:oa.upm.es:29085
DOI: 10.1039/c3sm27867k
Official URL: http://pubs.rsc.org/en/Content/ArticleLanding/2013/SM/c3sm27867k#!divAbstract
Deposited by: Memoria Investigacion
Deposited on: 26 Jun 2014 12:43
Last Modified: 22 Sep 2014 11:44
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