Building functional surfaces for biosensors development

Moron Fernandez, Carlos and Garcia Garcia, Alfonso and Tremps Guerra, Enrique and Somolinos Sanchez, Jose Andres (2013). Building functional surfaces for biosensors development. "Key Engineering Materials", v. 543 ; pp. 204-207. ISSN 1013-9826.


Title: Building functional surfaces for biosensors development
  • Moron Fernandez, Carlos
  • Garcia Garcia, Alfonso
  • Tremps Guerra, Enrique
  • Somolinos Sanchez, Jose Andres
Item Type: Article
Título de Revista/Publicación: Key Engineering Materials
Date: March 2013
Volume: 543
Freetext Keywords: Covalent binding, biosensor
Faculty: E.T.S.I. Navales (UPM)
Department: Sistemas Oceánicos y Navales [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Polyelectrolyte multilayers (PEM) built by layer-by-layer technique have been extensively studied over the last years, resulting in a wide variety of current and potential applications. This technique can be used to construct thin films with different functionalities, or to functionalize surfaces with substantial different properties of those of the underlying substrates. The multilayering process is achieved by the alternate adsorption of oppositely charged polyelectrolytes. In this work we get advantage of the protein resistant property of the Poly (l-lysine)-graft-(polyethyleneglycol) to create protein patterns. Proteins can be immobilized on a surface by unspecific physical adsorption, covalent binding or through specific interactions. The first protein used in this work was laccase, a copper-containing redox enzyme that catalyse the oxidation of a broad range of polyphenols and aromatic substrates, coupled to the reduction of O2 to H2O without need of cofactors. Applications of laccases have been reported in food, pulp, paper, and textile industry, and also in biosensor development. Some uses require the immobilization of the enzyme on solid supports by adsorption, covalent attachment, entrapment, etc, on several substrates. Especially for biosensor development, highly active, stable and reproducible immobilization of laccase is required.

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Item ID: 29146
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Deposited by: Memoria Investigacion
Deposited on: 14 Jul 2014 17:08
Last Modified: 22 Sep 2014 11:44
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