Nitrogen fixation by native Bradyrhizobia in symbiosis with Lupinus mariae-josephae requires a T3SS encoding a NopE-like effector

Duran Wendt, David Ricardo and Pastor Martín, Victor and Zehner, Susanne and Göttfert, Michael and Imperial Ródenas, Juan and Ruiz Argüeso, Tomas-Andres and Rey Navarro, Luis (2013). Nitrogen fixation by native Bradyrhizobia in symbiosis with Lupinus mariae-josephae requires a T3SS encoding a NopE-like effector. In: "XXXVI Congreso Sociedad Española de Bioquímica y Biología Molecular", 04/09/2013 - 06/09/2013, Madrid, Spain. pp. 254-255.

Description

Title: Nitrogen fixation by native Bradyrhizobia in symbiosis with Lupinus mariae-josephae requires a T3SS encoding a NopE-like effector
Author/s:
  • Duran Wendt, David Ricardo
  • Pastor Martín, Victor
  • Zehner, Susanne
  • Göttfert, Michael
  • Imperial Ródenas, Juan
  • Ruiz Argüeso, Tomas-Andres
  • Rey Navarro, Luis
Item Type: Presentation at Congress or Conference (Poster)
Event Title: XXXVI Congreso Sociedad Española de Bioquímica y Biología Molecular
Event Dates: 04/09/2013 - 06/09/2013
Event Location: Madrid, Spain
Title of Book: Nitrogen fixation by native Bradyrhizobia in symbiosis with Lupinus mariae-josephae requires a T3SS encoding a NopE-like effector
Date: 2013
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Several bradyrhizobial isolates from L. mariae-josephae root nodules [1] contain a type III secretion system (T3SS) within a cluster of about 30 genes. Among those genes, ttsI codes for the transcriptional activator of the system. Mutation of ttsI resulted in the formation of white, non-fixing nodules with the natural legume host, L. mariae-josephae. The T3SS cluster also contains a gene coding for a NopE-like protein. NopE proteins have been demonstrated to be effectors in the Bradyrhizobium-soybean symbiosis [2] and belong to a small group of poorly characterized proteins from plant-associated bacteria that contain one or two autocleavage motifs known as DUF1521 (Schirrmeister et al. 2011). The amino acid sequence of a NopE-like protein in the L. mariae-josephae strain LmjC contains just one autocatalytic motif. This is unlike NopE1 and NopE2 proteins secreted by the T3SS of B. japonicum, that contain two motifs [3]. The autocleavage of LmjC NopE protein was analyzed after expression in E. coli and purification. Two protein fragments of the predicted sizes appeared in the presence of Ca2+, Cu2+, Cd2+, Zn2+ and Mn2+ cations. In contrast, autocleavage did not take place in the presence of Ni2+, Co2+ or Mg2+. Site-directed mutagenesis of the DUF1521 motif in LmjC NopE abolished self-cleavage in vitro. Symbiotic competence of a NopE- mutant with the L. mariae-josephae host was not affected. Possible roles of NopE are discussed.

More information

Item ID: 29849
DC Identifier: http://oa.upm.es/29849/
OAI Identifier: oai:oa.upm.es:29849
Deposited by: Memoria Investigacion
Deposited on: 05 May 2015 15:55
Last Modified: 05 May 2015 15:55
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