MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis

Datt Pant, Bikram and Buhtz, Anja and Kehr, Julia and Scheible, Wolf-Rüdiger (2008). MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis. "Plant Journal", v. 53 (n. 5); pp. 731-738. ISSN 0960-7412. https://doi.org/10.1111/j.1365-313X.2007.03363.x.

Description

Title: MicroRNA399 is a long-distance signal for the regulation of plant phosphate homeostasis
Author/s:
  • Datt Pant, Bikram
  • Buhtz, Anja
  • Kehr, Julia
  • Scheible, Wolf-Rüdiger
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: March 2008
ISSN: 0960-7412
Volume: 53
Subjects:
Freetext Keywords: microRNA, phosphate homeostasis, long-distance signaling, nutrient, Arabidopsis, Brassica.
Faculty: Centro de Tecnología Biomédica (CTB) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The presence of microRNA species in plant phloem sap suggests potential signaling roles by long-distance lregulation of gene expression. Proof for such a role for a phloem-mobile microRNA is lacking. Here we show lthat phosphate (Pi) starvation-induced microRNA399 (miR399) is present in the phloem sap of two diverse lplant species, rapeseed and pumpkin, and levels are strongly and specifically increased in phloem sap during Pi ldeprivation. By performing micro-grafting experiments using Arabidopsis, we further show that chimeric lplants constitutively over-expressing miR399 in the shoot accumulate mature miR399 species to very high llevels in their wild-type roots, while corresponding primary transcripts are virtually absent in roots, ldemonstrating shoot-to-root transport. The chimeric plants exhibit (i) down-regulation of the miR399 target ltranscript (PHO2), which encodes a critical component for maintenance of Pi homeostasis, in the wild-type lroot, and (ii) Pi accumulation in the shoot, which is the phenotype of pho2 mutants, miR399 over-expressers or lchimeric plants with a genetic knock-out of PHO2 in the root. Hence the transported miR399 molecules retain lbiological activity. This is a demonstration of systemic control of a biological process, i.e. maintenance of plant lPi homeostasis, by a phloem-mobile microRNA.

More information

Item ID: 3035
DC Identifier: http://oa.upm.es/3035/
OAI Identifier: oai:oa.upm.es:3035
DOI: 10.1111/j.1365-313X.2007.03363.x
Official URL: http://www3.interscience.wiley.com/cgi-bin/fulltext/119410746/PDFSTART
Deposited by: Memoria Investigacion
Deposited on: 11 May 2010 11:07
Last Modified: 19 Feb 2019 15:54
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