Identification and functional characterization of Rhizobium leguminosarum bv. viciae genetic systems involved in nickel homeostasis.

Rubio Sanz, Laura; Prieto Carbajo, Rosa Isabel; Imperial Ródenas, Juan; Palacios Alberti, Jose Manuel y Brito Lopez, Maria Belen (2010). Identification and functional characterization of Rhizobium leguminosarum bv. viciae genetic systems involved in nickel homeostasis.. En: "XIII SEFIN. National Meeting of the Spanish Society of Nitrogen Fixation", 15/6/2010-18/6/2010, Zaragoza, España.

Descripción

Título: Identification and functional characterization of Rhizobium leguminosarum bv. viciae genetic systems involved in nickel homeostasis.
Autor/es:
  • Rubio Sanz, Laura
  • Prieto Carbajo, Rosa Isabel
  • Imperial Ródenas, Juan
  • Palacios Alberti, Jose Manuel
  • Brito Lopez, Maria Belen
Tipo de Documento: Ponencia en Congreso o Jornada (Artículo)
Título del Evento: XIII SEFIN. National Meeting of the Spanish Society of Nitrogen Fixation
Fechas del Evento: 15/6/2010-18/6/2010
Lugar del Evento: Zaragoza, España
Título del Libro: Biological Nitrogen Fixation and Plant-Associated Microorganisms
Fecha: 15 Junio 2010
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

Texto completo

[img]
Vista Previa
PDF (Document Portable Format) - Se necesita un visor de ficheros PDF, como GSview, Xpdf o Adobe Acrobat Reader
Descargar (30kB) | Vista Previa

Resumen

A collection of Rhizobium leguminosarum bv. viciae strains isolated from ultramafic and contaminated soils in Italy and Germany, respectively, was analyzed for resistance to nickel and cobalt ions. These assays led to the identification of strain UPM1137, which is able to grow at high concentrations of nickel and cobalt. In order to identify genetic systems involved in the homeostasis to these metals, a random mutagenesis was carried out in UPM1137 by inserting a Tn5-derivative minitransposon. As a result 4313 transconjugants were obtained, being 39 of them (0.90%) unable to grow at 1.5 mM NiCl2. The identification of the transposon insertion site in these mutants showed that the disrupted genes encode proteins belonging to different functional categories, where the secreted and membrane proteins were the most numerous. The analysis of heavy metal resistance and phenotypes in symbiotic and free –living cells will define the contribution of these genes to metal homeostasis.

Más información

ID de Registro: 30383
Identificador DC: http://oa.upm.es/30383/
Identificador OAI: oai:oa.upm.es:30383
URL Oficial: https://apps.eead.csic.es/EEAD/conference/13sefin/index.html
Depositado por: PhD Laura Rubio Sanz
Depositado el: 15 Jul 2014 12:28
Ultima Modificación: 22 Abr 2016 00:38
  • Open Access
  • Open Access
  • Sherpa-Romeo
    Compruebe si la revista anglosajona en la que ha publicado un artículo permite también su publicación en abierto.
  • Dulcinea
    Compruebe si la revista española en la que ha publicado un artículo permite también su publicación en abierto.
  • Recolecta
  • e-ciencia
  • Observatorio I+D+i UPM
  • OpenCourseWare UPM