Identification and functional characterization of Rhizobium leguminosarum bv. viciae genetic systems involved in nickel homeostasis.

Rubio Sanz, Laura and Prieto Carbajo, Rosa Isabel and Imperial Ródenas, Juan and Palacios Alberti, Jose Manuel and Brito Lopez, Maria Belen (2010). Identification and functional characterization of Rhizobium leguminosarum bv. viciae genetic systems involved in nickel homeostasis.. In: "XIII SEFIN. National Meeting of the Spanish Society of Nitrogen Fixation", 15/6/2010-18/6/2010, Zaragoza, España.

Description

Title: Identification and functional characterization of Rhizobium leguminosarum bv. viciae genetic systems involved in nickel homeostasis.
Author/s:
  • Rubio Sanz, Laura
  • Prieto Carbajo, Rosa Isabel
  • Imperial Ródenas, Juan
  • Palacios Alberti, Jose Manuel
  • Brito Lopez, Maria Belen
Item Type: Presentation at Congress or Conference (Article)
Event Title: XIII SEFIN. National Meeting of the Spanish Society of Nitrogen Fixation
Event Dates: 15/6/2010-18/6/2010
Event Location: Zaragoza, España
Title of Book: Biological Nitrogen Fixation and Plant-Associated Microorganisms
Date: 15 June 2010
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

A collection of Rhizobium leguminosarum bv. viciae strains isolated from ultramafic and contaminated soils in Italy and Germany, respectively, was analyzed for resistance to nickel and cobalt ions. These assays led to the identification of strain UPM1137, which is able to grow at high concentrations of nickel and cobalt. In order to identify genetic systems involved in the homeostasis to these metals, a random mutagenesis was carried out in UPM1137 by inserting a Tn5-derivative minitransposon. As a result 4313 transconjugants were obtained, being 39 of them (0.90%) unable to grow at 1.5 mM NiCl2. The identification of the transposon insertion site in these mutants showed that the disrupted genes encode proteins belonging to different functional categories, where the secreted and membrane proteins were the most numerous. The analysis of heavy metal resistance and phenotypes in symbiotic and free –living cells will define the contribution of these genes to metal homeostasis.

More information

Item ID: 30383
DC Identifier: http://oa.upm.es/30383/
OAI Identifier: oai:oa.upm.es:30383
Official URL: https://apps.eead.csic.es/EEAD/conference/13sefin/index.html
Deposited by: PhD Laura Rubio Sanz
Deposited on: 15 Jul 2014 12:28
Last Modified: 22 Apr 2016 00:38
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