Conservation of endangered Lupinus mariae-josephae in its natural habitat by inoculation with selected, native Bradyrhizobium strains

Navarro, Albert and Fos, Simón and Laguna Heras, Emilio and Duran Wendt, David and Rey Navarro, Luis and Rubio Sanz, Laura and Imperial Ródenas, Juan and Ruiz Argüeso, Tomas-Andres (2014). Conservation of endangered Lupinus mariae-josephae in its natural habitat by inoculation with selected, native Bradyrhizobium strains. "PloS One", v. 9 (n. 7); pp. 1-8. https://doi.org/10.1371/journal.pone.0102205.

Description

Title: Conservation of endangered Lupinus mariae-josephae in its natural habitat by inoculation with selected, native Bradyrhizobium strains
Author/s:
  • Navarro, Albert
  • Fos, Simón
  • Laguna Heras, Emilio
  • Duran Wendt, David
  • Rey Navarro, Luis
  • Rubio Sanz, Laura
  • Imperial Ródenas, Juan
  • Ruiz Argüeso, Tomas-Andres
Item Type: Article
Título de Revista/Publicación: PloS One
Date: 14 July 2014
Volume: 9
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Lupinus mariae-josephae is a recently discovered endemism that is only found in alkaline-limed soils, a unique habitat for lupines, from a small area in Valencia region (Spain). In these soils, L. mariae-josephae grows in just a few defined patches, and previous conservation efforts directed towards controlled plant reproduction have been unsuccessful. We have previously shown that L. mariae-josephae plants establish a specific root nodule symbiosis with bradyrhizobia present in those soils, and we reasoned that the paucity of these bacteria in soils might contribute to the lack of success in reproducing plants for conservation purposes. Greenhouse experiments using L. mariae-josephae trap-plants showed the absence or near absence of L. mariae-josephae-nodulating bacteria in ‘‘terra rossa’’ soils of Valencia outside of L. mariaejosephae plant patches, and in other ‘‘terra rossa’’ or alkaline red soils of the Iberian Peninsula and Balearic Islands outside of the Valencia L. mariae-josephae endemism region. Among the bradyrhizobia able to establish an efficient symbiosis with L. mariae-josephae plants, two strains, LmjC and LmjM3 were selected as inoculum for seed coating. Two planting experiments were carried out in consecutive years under natural conditions in areas with edapho-climatic characteristics identical to those sustaining natural L. mariae-josephae populations, and successful reproduction of the plant was achieved. Interestingly, the successful reproductive cycle was absolutely dependent on seedling inoculation with effective bradyrhizobia, and optimal performance was observed in plants inoculated with LmjC, a strain that had previously shown the most efficient behavior under controlled conditions. Our results define conditions for L. mariae-josephae conservation and for extension to alkaline-limed soil habitats, where no other known lupine can thrive.

More information

Item ID: 30652
DC Identifier: http://oa.upm.es/30652/
OAI Identifier: oai:oa.upm.es:30652
DOI: 10.1371/journal.pone.0102205
Official URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0102205
Deposited by: PhD Laura Rubio Sanz
Deposited on: 01 Aug 2014 08:42
Last Modified: 11 Apr 2019 11:34
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