Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Guzmán Delgado, Paula; Fernandez Fernandez, Victoria; Khayet, Mohamed; García, Maria Luisa; Fernández, Agustín y Gil Sanchez, Luis (2014). Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. "The Scientific World Journal" (n. 963921); pp. 1-9. https://doi.org/10.1155/2014/963921.

Descripción

Título: Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy
Autor/es:
  • Guzmán Delgado, Paula
  • Fernandez Fernandez, Victoria
  • Khayet, Mohamed
  • García, Maria Luisa
  • Fernández, Agustín
  • Gil Sanchez, Luis
Tipo de Documento: Artículo
Título de Revista/Publicación: The Scientific World Journal
Fecha: 22 Abril 2014
Materias:
Palabras Clave Informales: Embedding resin; Leaf; Plant cuticle; Solubility parameter; Transmission electron microscopy; Ultra-structure
Escuela: E.T.S.I. Montes, Forestal y del Medio Natural (UPM)
Departamento: Silvopascicultura [hasta 2014]
Licencias Creative Commons: Reconocimiento - No comercial

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Resumen

The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr’s resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.

Más información

ID de Registro: 30719
Identificador DC: http://oa.upm.es/30719/
Identificador OAI: oai:oa.upm.es:30719
Identificador DOI: 10.1155/2014/963921
URL Oficial: http://www.hindawi.com/journals/tswj/2014/963921/
Depositado por: Victoria Fernández
Depositado el: 25 Ago 2014 06:27
Ultima Modificación: 22 Abr 2016 00:59
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