Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy

Guzmán Delgado, Paula and Fernandez Fernandez, Victoria and Khayet, Mohamed and García, Maria Luisa and Fernández, Agustín and Gil Sanchez, Luis Alfonso (2014). Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy. "The Scientific World Journal" (n. 963921); pp. 1-9. https://doi.org/10.1155/2014/963921.

Description

Title: Ultrastructure of Plant Leaf Cuticles in relation to Sample Preparation as Observed by Transmission Electron Microscopy
Author/s:
  • Guzmán Delgado, Paula
  • Fernandez Fernandez, Victoria
  • Khayet, Mohamed
  • García, Maria Luisa
  • Fernández, Agustín
  • Gil Sanchez, Luis Alfonso
Item Type: Article
Título de Revista/Publicación: The Scientific World Journal
Date: 22 April 2014
Subjects:
Freetext Keywords: Embedding resin; Leaf; Plant cuticle; Solubility parameter; Transmission electron microscopy; Ultra-structure
Faculty: E.T.S.I. Montes, Forestal y del Medio Natural (UPM)
Department: Silvopascicultura [hasta 2014]
Creative Commons Licenses: Recognition - Non commercial

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Abstract

The leaf cuticular ultrastructure of some plant species has been examined by transmission electron microscopy (TEM) in only few studies. Attending to the different cuticle layers and inner structure, plant cuticles have been grouped into six general morphological types. With the aim of critically examining the effect of cuticle isolation and preparation for TEM analysis on cuticular ultrastructure, adaxial leaf cuticles of blue-gum eucalypt, grey poplar, and European pear were assessed, following a membrane science approach. The embedding and staining protocols affected the ultrastructure of the cuticles analysed. The solubility parameter, surface tension, and contact angles with water of pure Spurr's and LR-White resins were within a similar range. Differences were however estimated for resin : solvent mixtures, since Spurr’s resin is combined with acetone and LR-White resin is mixed with ethanol. Given the composite hydrophilic and lipophilic nature of plant cuticles, the particular TEM tissue embedding and staining procedures employed may affect sample ultrastructure and the interpretation of the results in physicochemical and biological terms. It is concluded that tissue preparation procedures may be optimised to facilitate the observation of the micro- and nanostructure of cuticular layers and components with different degrees of polarity and hydrophobicity.

More information

Item ID: 30719
DC Identifier: http://oa.upm.es/30719/
OAI Identifier: oai:oa.upm.es:30719
DOI: 10.1155/2014/963921
Official URL: http://www.hindawi.com/journals/tswj/2014/963921/
Deposited by: Victoria Fernández
Deposited on: 25 Aug 2014 06:27
Last Modified: 10 Jan 2019 13:03
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