The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background

Miguens Vázquez, Miguel; Kastanauskaite, Asta; Coria, Santiago M.; Selvas, Abraham; Ballesteros-Yañez, Inmaculada; Felipe Oroquieta, Javier de y Ambrosio, Emilio (2015). The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background. "Cerebral Cortex", v. 25 (n. 1); pp. 56-65. ISSN 1047-3211. https://doi.org/10.1093/cercor/bht200.

Descripción

Título: The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background
Autor/es:
  • Miguens Vázquez, Miguel
  • Kastanauskaite, Asta
  • Coria, Santiago M.
  • Selvas, Abraham
  • Ballesteros-Yañez, Inmaculada
  • Felipe Oroquieta, Javier de
  • Ambrosio, Emilio
Tipo de Documento: Artículo
Título de Revista/Publicación: Cerebral Cortex
Fecha: Enero 2015
Volumen: 25
Materias:
Palabras Clave Informales: CA1, drugs of abuse, Fischer 344, Lewis, pyramidal neuron
Escuela: Centro de Tecnología Biomédica (CTB) (UPM)
Departamento: Otro
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Chronic exposure to cocaine induces modifications to neurons in the brain regions involved in addiction. Hence, we evaluated cocaine-induced changes in the hippocampal CA1 field in Fischer 344 (F344) and Lewis (LEW) rats, 2 strains that have been widely used to study genetic predisposition to drug addiction, by combining intracellular Lucifer yellow injection with confocal microscopy reconstruction of labeled neurons. Specifically, we examined the effects of cocaine self-administration on the structure, size, and branching complexity of the apical dendrites of CA1 pyramidal neurons. In addition, we quantified spine density in the collaterals of the apical dendritic arbors of these neurons. We found differences between these strains in several morphological parameters. For example, CA1 apical dendrites were more branched and complex in LEW than in F344 rats, while the spine density in the collateral dendrites of the apical dendritic arbors was greater in F344 rats. Interestingly, cocaine self-administration in LEW rats augmented the spine density, an effect that was not observed in the F344 strain. These results reveal significant structural differences in CA1 pyramidal cells between these strains and indicate that cocaine self-administration has a distinct effect on neuron morphology in the hippocampus of rats with different genetic backgrounds.

Más información

ID de Registro: 32284
Identificador DC: http://oa.upm.es/32284/
Identificador OAI: oai:oa.upm.es:32284
Identificador DOI: 10.1093/cercor/bht200
URL Oficial: http://cercor.oxfordjournals.org/content/25/1/56.abstract
Depositado por: Memoria Investigacion
Depositado el: 21 Abr 2015 15:52
Ultima Modificación: 01 Feb 2016 23:56
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