The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background

Miguens Vázquez, Miguel and Kastanauskaite, Asta and Coria, Santiago M. and Selvas, Abraham and Ballesteros-Yañez, Inmaculada and Felipe Oroquieta, Javier de and Ambrosio, Emilio (2015). The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background. "Cerebral Cortex", v. 25 (n. 1); pp. 56-65. ISSN 1047-3211. https://doi.org/10.1093/cercor/bht200.

Description

Title: The effects of cocaine self-administration on dendritic spine density in the rat hippocampus are dependent on genetic background
Author/s:
  • Miguens Vázquez, Miguel
  • Kastanauskaite, Asta
  • Coria, Santiago M.
  • Selvas, Abraham
  • Ballesteros-Yañez, Inmaculada
  • Felipe Oroquieta, Javier de
  • Ambrosio, Emilio
Item Type: Article
Título de Revista/Publicación: Cerebral Cortex
Date: January 2015
ISSN: 1047-3211
Volume: 25
Subjects:
Freetext Keywords: CA1, drugs of abuse, Fischer 344, Lewis, pyramidal neuron
Faculty: Centro de Tecnología Biomédica (CTB) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Chronic exposure to cocaine induces modifications to neurons in the brain regions involved in addiction. Hence, we evaluated cocaine-induced changes in the hippocampal CA1 field in Fischer 344 (F344) and Lewis (LEW) rats, 2 strains that have been widely used to study genetic predisposition to drug addiction, by combining intracellular Lucifer yellow injection with confocal microscopy reconstruction of labeled neurons. Specifically, we examined the effects of cocaine self-administration on the structure, size, and branching complexity of the apical dendrites of CA1 pyramidal neurons. In addition, we quantified spine density in the collaterals of the apical dendritic arbors of these neurons. We found differences between these strains in several morphological parameters. For example, CA1 apical dendrites were more branched and complex in LEW than in F344 rats, while the spine density in the collateral dendrites of the apical dendritic arbors was greater in F344 rats. Interestingly, cocaine self-administration in LEW rats augmented the spine density, an effect that was not observed in the F344 strain. These results reveal significant structural differences in CA1 pyramidal cells between these strains and indicate that cocaine self-administration has a distinct effect on neuron morphology in the hippocampus of rats with different genetic backgrounds.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainPND-2012I057UnspecifiedUnspecifiedUnspecified
Government of SpainBFU2012–34963UnspecifiedUnspecifiedUnspecified

More information

Item ID: 32284
DC Identifier: http://oa.upm.es/32284/
OAI Identifier: oai:oa.upm.es:32284
DOI: 10.1093/cercor/bht200
Official URL: http://cercor.oxfordjournals.org/content/25/1/56.abstract
Deposited by: Memoria Investigacion
Deposited on: 21 Apr 2015 15:52
Last Modified: 30 May 2019 14:03
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