Citation
Ortega González, Francisco Javier and Sanza Gutiérrez, Francisco Javier and Laguna Heras, Maria Fe and Holgado Bolaños, Miguel and Casquel del Campo, Rafael and Bañuls, María José and Angulo Barrios, Carlos and López-Romero Moraleda, David and Maquieira, Ángel and Puchades, Rosa
(2013).
Development of a versatile biotinylated material based on SU-8.
"Journal of Materials Chemistry B", v. 1
(n. 21);
pp. 2750-2756.
ISSN 2050-750X.
https://doi.org/10.1039/c3tb20323a.
Abstract
The negative epoxy-based SU-8 photoresist has a wide variety of applications within the semiconductor industry, photonics and lab-on-a-chip devices, and it is emerging as an alternative to silicon-based devices for sensing purposes. In the present work, biotinylation of the SU-8 polymer surface promoted by light is reported. As a result, a novel, efective, and low-cost material, focusing on the immobilization of bioreceptors and consequent biosensing, is developed. This material allows the spatial discrimination depending on the irradiation of desired areas. The most salient feature is that the photobiotin may be directly incorporated into the SU-8 curing process, consequently reducing time and cost. The potential use of this substrate is demonstrated by the immunoanalytical detection of the synthetic steroid gestrinone, showing excellent performances. Moreover, the naked eye biodetection due to the transparent SU-8 substrate, and simple instrumental quantication are additional advantages.
