Technical note: Comparison of automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) to assess bacterial diversity in the rumen of sheep

Saro, C. and Ranilla, M. J. and Cifuentes, A. and Rosselló-Mora, R. and Carro Travieso, María Dolores (2014). Technical note: Comparison of automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) to assess bacterial diversity in the rumen of sheep. "Journal of Animal Science", v. 92 (n. 3); pp. 1083-1088. ISSN 0021-8812. https://doi.org/10.2527/jas.2013-7175.

Description

Title: Technical note: Comparison of automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) to assess bacterial diversity in the rumen of sheep
Author/s:
  • Saro, C.
  • Ranilla, M. J.
  • Cifuentes, A.
  • Rosselló-Mora, R.
  • Carro Travieso, María Dolores
Item Type: Article
Título de Revista/Publicación: Journal of Animal Science
Date: November 2014
ISSN: 0021-8812
Volume: 92
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The aim of this study was to compare automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques to assess bacterial diversity in the rumen of sheep. Sheep were fed 2 diets with 70% of either alfalfa hay or grass hay, and the solid (SOL) and liquid (LIQ) phases of the rumen were sampled immediately before feeding (0 h) and at 4 and 8 h postfeeding. Both techniques detected similar differences between forages, with alfalfa hay promoting greater (P < 0.05) bacterial diversity than grass hay. In contrast, whereas ARISA analysis showed a decrease (P < 0.05) of bacterial diversity in SOL at 4 h postfeeding compared with 0 and 8 h samplings, no variations (P > 0.05) over the postfeeding period were detected by DGGE. The ARISA technique showed lower (P < 0.05) bacterial diversity in SOL than in LIQ samples at 4 h postfeeding, but no differences (P > 0.05) in bacterial diversity between both rumen phases were detected by DGGE. Under the conditions of this study, the DGGE was not sensitive enough to detect some changes in ruminal bacterial communities, and therefore ARISA was considered more accurate for assessing bacterial diversity of ruminal samples. The results highlight the influence of the fingerprinting technique used to draw conclusions on factors affecting ruminal bacterial diversity.

More information

Item ID: 35233
DC Identifier: http://oa.upm.es/35233/
OAI Identifier: oai:oa.upm.es:35233
DOI: 10.2527/jas.2013-7175
Official URL: https://www.animalsciencepublications.org/publications/jas/abstracts/92/3/1083?search-result=1
Deposited by: Memoria Investigacion
Deposited on: 28 May 2015 16:42
Last Modified: 28 May 2015 16:42
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