Induction of ovulation in rabbits by adding Lecirelin to the seminal dose: In vitro and in vivo effects of different excipients.

Dal Bosco, A. and Cardinali, R. and Brecchia, G. and Garcia Rebollar, Pilar and Fatnassi, M. and Millán, P. and Mattioli, S. and Castellini, C. (2014). Induction of ovulation in rabbits by adding Lecirelin to the seminal dose: In vitro and in vivo effects of different excipients.. "Animal Reproduction Science", v. 150 (n. 1-2); pp. 44-49. ISSN 0378-4320. https://doi.org/10.1016/j.anireprosci.2014.08.009.

Description

Title: Induction of ovulation in rabbits by adding Lecirelin to the seminal dose: In vitro and in vivo effects of different excipients.
Author/s:
  • Dal Bosco, A.
  • Cardinali, R.
  • Brecchia, G.
  • Garcia Rebollar, Pilar
  • Fatnassi, M.
  • Millán, P.
  • Mattioli, S.
  • Castellini, C.
Item Type: Article
Título de Revista/Publicación: Animal Reproduction Science
Date: November 2014
ISSN: 0378-4320
Volume: 150
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview

Abstract

This study evaluates the effect of Lecirelin (Dalmarelin®, Fatro, Italy) diluted in different excipients (benzilic alcohol, benzoic acid and paraben) added to a seminal dose on LH concentrations, progesterone concentrations and ovarian status in rabbits. The in vitro effect on spermatozoa was also tested. A total of 100 multiparous female rabbits were divided into 5 groups, which at the moment of AI, received 0.2 mL (5 μg/dose) intramuscular (im) inoculation of Lecirelin (control) or the same Lecirelin dose administered intravaginally (iv) with the seminal dose alone (Lecirelin group) or with benzilic alcohol (Lecirelin BA group), benzoic acid (Lecirelin BAc group) or parabens (Lecirelin PA group) as an excipient. After 7 days, 10 rabbits per group were euthanized to analyze their ovarian status. In the control group, a high LH peak was detected 30 min post AI, while in the iv groups a slight increase in LH occurred after 120 min. The ovulation and fertility rate was similar in control and Lecirelin groups, while the lowest fertility rate was detected in the Lecirelin BA group. In a second experiment, the semen samples collected from male rabbits were diluted in TALP (control) or mixed with the 5 μg of Lecirelin solutions used in the first experiment. The highest percentage of capacitated sperm (68.3%) was recorded in the Lecirelin PA. The lowest percentages were observed in the Lecirelin BA and BAc groups. In conclusion, the iv administration of Lecirelin represents an alternative method for simplifying rabbit insemination procedures.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL-2011-23822UnspecifiedUnspecifiedUnspecified

More information

Item ID: 35241
DC Identifier: http://oa.upm.es/35241/
OAI Identifier: oai:oa.upm.es:35241
DOI: 10.1016/j.anireprosci.2014.08.009
Official URL: http://www.sciencedirect.com/science/article/pii/S0378432014002619
Deposited by: Memoria Investigacion
Deposited on: 01 Jun 2015 12:07
Last Modified: 08 May 2019 06:34
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM