Induction of ovulation in rabbits by adding Lecirelin to the seminal dose: In vitro and in vivo effects of different excipients.

Dal Bosco, A.; Cardinali, R.; Brecchia, G.; Garcia Rebollar, Pilar; Fatnassi, M.; Millán, P.; Mattioli, S. y Castellini, C. (2014). Induction of ovulation in rabbits by adding Lecirelin to the seminal dose: In vitro and in vivo effects of different excipients.. "Animal Reproduction Science", v. 150 (n. 1-2); pp. 44-49. ISSN 0378-4320. https://doi.org/10.1016/j.anireprosci.2014.08.009.

Descripción

Título: Induction of ovulation in rabbits by adding Lecirelin to the seminal dose: In vitro and in vivo effects of different excipients.
Autor/es:
  • Dal Bosco, A.
  • Cardinali, R.
  • Brecchia, G.
  • Garcia Rebollar, Pilar
  • Fatnassi, M.
  • Millán, P.
  • Mattioli, S.
  • Castellini, C.
Tipo de Documento: Artículo
Título de Revista/Publicación: Animal Reproduction Science
Fecha: Noviembre 2014
Volumen: 150
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Producción Agraria
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

This study evaluates the effect of Lecirelin (Dalmarelin®, Fatro, Italy) diluted in different excipients (benzilic alcohol, benzoic acid and paraben) added to a seminal dose on LH concentrations, progesterone concentrations and ovarian status in rabbits. The in vitro effect on spermatozoa was also tested. A total of 100 multiparous female rabbits were divided into 5 groups, which at the moment of AI, received 0.2 mL (5 μg/dose) intramuscular (im) inoculation of Lecirelin (control) or the same Lecirelin dose administered intravaginally (iv) with the seminal dose alone (Lecirelin group) or with benzilic alcohol (Lecirelin BA group), benzoic acid (Lecirelin BAc group) or parabens (Lecirelin PA group) as an excipient. After 7 days, 10 rabbits per group were euthanized to analyze their ovarian status. In the control group, a high LH peak was detected 30 min post AI, while in the iv groups a slight increase in LH occurred after 120 min. The ovulation and fertility rate was similar in control and Lecirelin groups, while the lowest fertility rate was detected in the Lecirelin BA group. In a second experiment, the semen samples collected from male rabbits were diluted in TALP (control) or mixed with the 5 μg of Lecirelin solutions used in the first experiment. The highest percentage of capacitated sperm (68.3%) was recorded in the Lecirelin PA. The lowest percentages were observed in the Lecirelin BA and BAc groups. In conclusion, the iv administration of Lecirelin represents an alternative method for simplifying rabbit insemination procedures.

Más información

ID de Registro: 35241
Identificador DC: http://oa.upm.es/35241/
Identificador OAI: oai:oa.upm.es:35241
Identificador DOI: 10.1016/j.anireprosci.2014.08.009
URL Oficial: http://www.sciencedirect.com/science/article/pii/S0378432014002619
Depositado por: Memoria Investigacion
Depositado el: 01 Jun 2015 12:07
Ultima Modificación: 30 Nov 2015 23:56
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